Abstract

TRD 4: Next-generation nanoprobe toolkit for biomedical applications Investigators: Moungi Bawendi (MIT) (4.1), Ishan Barman (JHU) (4.2), Conor Evans (MGH) (4.2,4.3), Gabriela Schlau-Cohen (MIT) (4.3) Collaborative Projects: Rakesh Jain (MGH) (CP1), Saraswati Sukumar, Johns Hopkins (CP10); PnP Research Corporation(CP7), Matthew Coleman, UC Davis (CP9). This TRD adds a synergistic focus on the development of novel molecular probes to complement and enhance the strong spectroscopy, imaging hardware and label-free assay developments of the LBRC. Molecular probes, tailored to our hardware development projects provide powerful toolkits that meet the demands of our collaborators for ultrasensitive, gene- and protein-specific analysis. We seek to develop nanostructured probes that enable new modes of visualization of biological function in real time. We are pulled by our collaborators to develop engineered nanoprobes to study metastasis and treatment response (Jain, CP1), to understand epigenetics factors (Sukumar, CP10), and to optimize cancer therapeutics (Coleman, CP9; PnP Research Corporation, CP7). We will leverage our bright nanoparticles (NPs) in combination with our advances in the other TRDs to improve our understanding, diagnosis, and monitoring of critical pathological conditions. Ultimately, conjugation of these nanoprobes to targeting entities will enable facile detection of numerous orthogonal biological processes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Biotechnology Resource Grants (P41)
Project #
5P41EB015871-34
Application #
9985832
Study Section
Special Emphasis Panel (ZEB1)
Project Start
1997-06-01
Project End
2022-05-31
Budget Start
2020-06-01
Budget End
2021-05-31
Support Year
34
Fiscal Year
2020
Total Cost
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02142

  Publications

Xue, Yi; So, Peter T C (2018) Three-dimensional super-resolution high-throughput imaging by structured illumination STED microscopy. Opt Express 26:20920-20928
Pandey, Rishikesh; Singh, Surya P; Zhang, Chi et al. (2018) Label-free spectrochemical probe for determination of hemoglobin glycation in clinical blood samples. J Biophotonics 11:e201700397
Carr, Jessica A; Franke, Daniel; Caram, Justin R et al. (2018) Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green. Proc Natl Acad Sci U S A 115:4465-4470
Quinn, Steven D; Srinivasan, Shwetha; Gordon, Jesse B et al. (2018) Single-Molecule Fluorescence Detection of the Epidermal Growth Factor Receptor in Membrane Discs. Biochemistry :
Jin, Di; Zhou, Renjie; Yaqoob, Zahid et al. (2018) Dynamic spatial filtering using a digital micromirror device for high-speed optical diffraction tomography. Opt Express 26:428-437
Yannas, Ioannis V; Tzeranis, Dimitrios S; So, Peter T C (2018) Regeneration mechanism for skin and peripheral nerves clarified at the organ and molecular scales. Curr Opin Biomed Eng 6:1-7
Hosseini, Poorya; Jin, Di; Yaqoob, Zahid et al. (2018) Single-shot dual-wavelength interferometric microscopy. Methods 136:35-39
Carr, Jessica A; Aellen, Marianne; Franke, Daniel et al. (2018) Absorption by water increases fluorescence image contrast of biological tissue in the shortwave infrared. Proc Natl Acad Sci U S A 115:9080-9085
Jonas, Oliver; Kang, Jeon Woong; Singh, Surya P et al. (2018) In vivo detection of drug-induced apoptosis in tumors using Raman spectroscopy. Analyst 143:4836-4839
Bartelt, Alexander; Widenmaier, Scott B; Schlein, Christian et al. (2018) Brown adipose tissue thermogenic adaptation requires Nrf1-mediated proteasomal activity. Nat Med 24:292-303

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