2. TRD#1: New Approaches to Specimen Preparation Abstract We are developing novel methods for specimen preparation that provide several advantages over traditional methods. These advantages include consumption of very small amounts of sample, and providing for more control over the quality of the prepared grids. The system uses a robotic device, called Spotiton, to dispense drops with volumes on the order of nanoliters to precise locations on an EM grid. The success of the device is coupled to a new ?self-blotting? grid that we have developed to provide a method for spreading sample to a thin film without the use of externally applied filter paper. Over the next 5 years we will design, develop, test and validate a generalized Spotiton system capable of multiple tasks including making well controlled vitrified grids, managing time resolved and fast mixing experiments, and preparing ~100 independent negatively stained samples on a single grid. We anticipate that a version of this device will be manufactured by a commercial company so that it becomes generally available to the research community.
Li, Yunlong; Sharma, Manjuli R; Koripella, Ravi K et al. (2018) Zinc depletion induces ribosome hibernation in mycobacteria. Proc Natl Acad Sci U S A 115:8191-8196 |
McGoldrick, Luke L; Singh, Appu K; Saotome, Kei et al. (2018) Opening of the human epithelial calcium channel TRPV6. Nature 553:233-237 |
Oliveira, Lucas M; Ye, Ze; Katz, Al et al. (2018) Component tree analysis of cystovirus ?6 nucleocapsid Cryo-EM single particle reconstructions. PLoS One 13:e0188858 |
Rheinberger, Jan; Gao, Xiaolong; Schmidpeter, Philipp Am et al. (2018) Ligand discrimination and gating in cyclic nucleotide-gated ion channels from apo and partial agonist-bound cryo-EM structures. Elife 7: |
Singh, Appu K; McGoldrick, Luke L; Sobolevsky, Alexander I (2018) Structure and gating mechanism of the transient receptor potential channel TRPV3. Nat Struct Mol Biol 25:805-813 |
Kang, Jin Young; Mooney, Rachel Anne; Nedialkov, Yuri et al. (2018) Structural Basis for Transcript Elongation Control by NusG Family Universal Regulators. Cell 173:1650-1662.e14 |
Benoit, Matthieu P M H; Asenjo, Ana B; Sosa, Hernando (2018) Cryo-EM reveals the structural basis of microtubule depolymerization by kinesin-13s. Nat Commun 9:1662 |
Sun, Yadong; Zhang, Yixiao; Hamilton, Keith et al. (2018) Molecular basis for the recognition of the human AAUAAA polyadenylation signal. Proc Natl Acad Sci U S A 115:E1419-E1428 |
Wei, Hui; Dandey, Venkata P; Zhang, Zhening et al. (2018) Optimizing ""self-wicking"" nanowire grids. J Struct Biol 202:170-174 |
Piasecka, Anna; Czapinska, Honorata; Vielberg, Marie-Theres et al. (2018) The Y. bercovieri Anbu crystal structure sheds light on the evolution of highly (pseudo)symmetric multimers. J Mol Biol 430:611-627 |
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