Abstract

Since its inception the Yeast Resource Center has specialized in extracting information about protein function from genome sequence. We began when the first eukaryotic genome was completed (budding yeast) and developed an array of technologies to decipher protein function from genome sequence. In this application, we make the natural progression towards understanding how protein variation affects protein levels, modification, function and structure. We propose to develop technologies in four areas: 1) To identify different protein variants or isoforms. 2) To quantify proteins by mass spectrometry with a focus on determining absolute levels of a protein. 3) To study how protein variation affects protein function. 4) To determine the structures of protein complexes not amenable to high-resolution methods such as x-ray crystallography and NMR.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Biotechnology Resource Grants (P41)
Project #
3P41GM103533-20S2
Application #
9422225
Study Section
Special Emphasis Panel (ZRG1-CB-L (40)P)
Program Officer
Sheeley, Douglas
Project Start
1997-09-30
Project End
2017-06-30
Budget Start
2015-07-01
Budget End
2017-06-30
Support Year
20
Fiscal Year
2017
Total Cost
$470,903
Indirect Cost
$129,214

  Institution

Name
University of Washington
Department
Biochemistry
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

DaRosa, Paul A; Harrison, Joseph S; Zelter, Alex et al. (2018) A Bifunctional Role for the UHRF1 UBL Domain in the Control of Hemi-methylated DNA-Dependent Histone Ubiquitylation. Mol Cell 72:753-765.e6
Xu, Yi; Ju, Ho-Jong; DeBlasio, Stacy et al. (2018) A Stem-Loop Structure in Potato Leafroll Virus Open Reading Frame 5 (ORF5) Is Essential for Readthrough Translation of the Coat Protein ORF Stop Codon 700 Bases Upstream. J Virol 92:
Burke, Jordan E; Longhurst, Adam D; Merkurjev, Daria et al. (2018) Spliceosome Profiling Visualizes Operations of a Dynamic RNP at Nucleotide Resolution. Cell 173:1014-1030.e17
Xavier, Marina Amaral; Tirloni, Lucas; Pinto, Antônio F M et al. (2018) A proteomic insight into vitellogenesis during tick ovary maturation. Sci Rep 8:4698
Hollmann, Taylor; Kim, Tae Kwon; Tirloni, Lucas et al. (2018) Identification and characterization of proteins in the Amblyomma americanum tick cement cone. Int J Parasitol 48:211-224
Mutlu, Beste; Chen, Huei-Mei; Moresco, James J et al. (2018) Regulated nuclear accumulation of a histone methyltransferase times the onset of heterochromatin formation in C. elegans embryos. Sci Adv 4:eaat6224
Jester, Benjamin W; Tinberg, Christine E; Rich, Matthew S et al. (2018) Engineered Biosensors from Dimeric Ligand-Binding Domains. ACS Synth Biol 7:2457-2467
Kim, Yeoun Jin; Sweet, Steve M; Egertson, Jarrett D et al. (2018) Data-independent acquisition mass spectrometry to quantify protein levels in FFPE tumor biopsies for molecular diagnostics. J Proteome Res :
Keich, Uri; Noble, William Stafford (2018) Controlling the FDR in imperfect matches to an incomplete database. J Am Stat Assoc 113:973-982
Louka, Panagiota; Vasudevan, Krishna Kumar; Guha, Mayukh et al. (2018) Proteins that control the geometry of microtubules at the ends of cilia. J Cell Biol 217:4298-4313

Showing the most recent 10 out of 372 publications