Abstract

TR&D3 - Experimental design in solid-state NMR spectroscopy Summary High resolution MAS-based SSNMR can be used to obtain detailed site-specific information on the structure, thermodynamics and dynamics of biopolymers, and is applicable to a broad range of systems that in native form are inaccessible to other methods. Solution and solid-state NMR are complementary to each other, and to X-ray diffraction and cryo electron microscopy, in terms of many methodological aspects and in terms of biological opportunities. TR&D3 at CoMD/NMR will utilize the strong expertise and instrumentation at NYSBC to develop technology for investigation of conformational dynamics of biological macromolecules by solid-state NMR spectroscopy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Biotechnology Resource Grants (P41)
Project #
5P41GM118302-03
Application #
9710696
Study Section
Special Emphasis Panel (ZRG1)
Project Start
Project End
Budget Start
2019-06-01
Budget End
2020-05-31
Support Year
3
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
New York Structural Biology Center
Department
Type
DUNS #
011191520
City
New York
State
NY
Country
United States
Zip Code
10027

  Publications

Hsu, Andrew; O'Brien, Paul A; Bhattacharya, Shibani et al. (2018) Enhanced spectral density mapping through combined multiple-field deuterium 13CH2D methyl spin relaxation NMR spectroscopy. Methods 138-139:76-84
Koss, Hans; Rance, Mark; Palmer 3rd, Arthur G (2018) General Expressions for Carr-Purcell-Meiboom-Gill Relaxation Dispersion for N-Site Chemical Exchange. Biochemistry 57:4753-4763
Hsu, Andrew; Ferrage, Fabien; Palmer 3rd, Arthur G (2018) Analysis of NMR Spin-Relaxation Data Using an Inverse Gaussian Distribution Function. Biophys J 115:2301-2309
Vugmeyster, Liliya; Griffin, Aaron; Ostrovsky, Dmitry et al. (2018) Correlated motions of C'-N and C?-C? pairs in protonated and per-deuterated GB3. J Biomol NMR 72:39-54
O'Brien, Paul A; Palmer 3rd, Arthur G (2018) TROSY pulse sequence for simultaneous measurement of the 15N R1 and {1H}-15N NOE in deuterated proteins. J Biomol NMR 70:205-209
Fritzsching, Keith J; Itin, Boris; McDermott, Ann E (2018) N,N-Diethylmethylamine as lineshape standard for NMR above 130?K. J Magn Reson 287:110-112
Rogawski, Rivkah; Sergeyev, Ivan V; Zhang, Yinglu et al. (2017) NMR Signal Quenching from Bound Biradical Affinity Reagents in DNP Samples. J Phys Chem B 121:10770-10781
Rogawski, Rivkah; McDermott, Ann E (2017) New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR. Arch Biochem Biophys 628:102-113