With success of the sequencing projects, the structural characterization of the genome-encoded proteins becomes increasingly important. Extensive knowledge of protein structure will significantly aid the investigation of protein function, protein interactions, and biochemical pathways. It will also have a major impact on our understanding of biology, human disease, and eventually on drug design. Experimental determination of structure is inherently time-consuming and costly, and currently there is a two orders-of- magnitude gap in numbers between sequencing and structure determination efforts. Computational techniques of structure modeling and prediction hold great promise for narrowing this gap. The Critical Assessment of Protein Structure Prediction (CASP) process was established to answer two questions: First, what level of prediction quality can be expected of these techniques? And second, which methods offer the best prospects for continued development? CASP is a community-wide program, with 98 research groups worldwide submitting over 3,800 predictions in the last round. Our group is the primary infrastructure resource for CASP, and handles processing of predictions, develops and implements evaluation software, performs prediction assessment, develops analysis and display tools, and facilitates access to predictions and their evaluation data. The primary goal of this project is to provide the infrastructure for the CASP prediction experiments. We propose to support the continuing operation of CASP and to expand its infrastructure, including an increased capacity for assessing predictions, further development and refinement of the evaluation criteria and software, and improved prediction analysis methods. We will also implement a continuous web-based mechanism for evaluation of prediction methods, based on structural genomics prediction targets. With this mechanism in place, we will be able to evaluate prediction methods on targets that are relevant to the interpretation of the genome sequence data, and record progress in structure prediction essentially in parallel with progress in structural genomics.

Agency
National Institute of Health (NIH)
Institute
National Library of Medicine (NLM)
Type
Biotechnology Resource Grants (P41)
Project #
1P41LM007085-01
Application #
6230045
Study Section
Biomedical Library and Informatics Review Committee (BLR)
Program Officer
Florance, Valerie
Project Start
2001-03-01
Project End
2004-02-29
Budget Start
2001-03-01
Budget End
2002-02-28
Support Year
1
Fiscal Year
2001
Total Cost
$454,961
Indirect Cost
Name
Lawrence Livermore National Laboratory
Department
Biology
Type
Organized Research Units
DUNS #
827171463
City
Livermore
State
CA
Country
United States
Zip Code
94550
Monastyrskyy, Bohdan; Fidelis, Krzysztof; Tramontano, Anna et al. (2011) Evaluation of residue-residue contact predictions in CASP9. Proteins 79 Suppl 10:119-25
Kryshtafovych, Andriy; Fidelis, Krzysztof; Tramontano, Anna (2011) Evaluation of model quality predictions in CASP9. Proteins 79 Suppl 10:91-106
Kryshtafovych, Andriy; Fidelis, Krzysztof; Moult, John (2011) CASP9 results compared to those of previous CASP experiments. Proteins 79 Suppl 10:196-207
Monastyrskyy, Bohdan; Fidelis, Krzysztof; Moult, John et al. (2011) Evaluation of disorder predictions in CASP9. Proteins 79 Suppl 10:107-18
Kryshtafovych, Andriy; Moult, John; Bartual, Sergio G et al. (2011) Target highlights in CASP9: Experimental target structures for the critical assessment of techniques for protein structure prediction. Proteins 79 Suppl 10:6-20
Kryshtafovych, Andriy; Krysko, Oleh; Daniluk, Pawel et al. (2009) Protein structure prediction center in CASP8. Proteins 77 Suppl 9:5-9
Kryshtafovych, Andriy; Fidelis, Krzysztof; Moult, John (2009) CASP8 results in context of previous experiments. Proteins 77 Suppl 9:217-28
Hvidsten, Torgeir R; Laegreid, Astrid; Kryshtafovych, Andriy et al. (2009) A comprehensive analysis of the structure-function relationship in proteins based on local structure similarity. PLoS One 4:e6266
Bjorkholm, Patrik; Daniluk, Pawel; Kryshtafovych, Andriy et al. (2009) Using multi-data hidden Markov models trained on local neighborhoods of protein structure to predict residue-residue contacts. Bioinformatics 25:1264-70
Moult, John; Fidelis, Krzysztof; Kryshtafovych, Andriy et al. (2009) Critical assessment of methods of protein structure prediction - Round VIII. Proteins 77 Suppl 9:1-4

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