This year we have experienced more down time than for about 20 years. In January, 1998, James Kremer, our former Chief of Operations, gave two weeks notice that he would be leaving for a job in the software industry. Jan 30 was his last day at our facility; that day the high voltage circuit went down. We got it running at 500KV and continued to operate at that voltage until April 14, when the filament circuit went out. In early February we experienced difficulties with control of the objective aperture, but a temporary repair by Mastronarde and Morphew got us working until electronic problems shut us down. In February we advertised nationally for a new person to maintain the microscope. Twenty applications were received and the five most promising were interviewed. Our top choice was Mr. David Rinaldi, who was then working as a field engineer for JEOL (the manufacturer of our HVEM), and he had already had experience working on the HT tank of a 1000KV JEOL microscope at the National Microscope Lab. in Berkeley, CA. He accepted our offer on March 18 and began work on April 6. Although Kremer came in on several occasions to help Rinaldi get started on our scope (which is substantially older than the one with which he had experience), Rinaldi has been largely on his own. This has made his job difficult, and getting the scope working has taken much longer than we had hoped. Problems with old components in need of replacement, and significant lag times for parts delivery, have plagued our getting going again. As of September 28, we are able to operate the microscope again at 1000KV. There are minor beam instabilities which may imrpove with better alignment or may simply require time to dissipate; we are currently assessing whether these instabilities affect image quality.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR000592-29
Application #
6117477
Study Section
Project Start
1998-12-01
Project End
1999-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
29
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Colorado at Boulder
Department
Type
DUNS #
City
Boulder
State
CO
Country
United States
Zip Code
80309
Giddings Jr, Thomas H; Morphew, Mary K; McIntosh, J Richard (2017) Preparing Fission Yeast for Electron Microscopy. Cold Spring Harb Protoc 2017:
Zhao, Xiaowei; Schwartz, Cindi L; Pierson, Jason et al. (2017) Three-Dimensional Structure of the Ultraoligotrophic Marine Bacterium ""Candidatus Pelagibacter ubique"". Appl Environ Microbiol 83:
Brown, Joanna R; Schwartz, Cindi L; Heumann, John M et al. (2016) A detailed look at the cytoskeletal architecture of the Giardia lamblia ventral disc. J Struct Biol 194:38-48
Saheki, Yasunori; Bian, Xin; Schauder, Curtis M et al. (2016) Control of plasma membrane lipid homeostasis by the extended synaptotagmins. Nat Cell Biol 18:504-15
Höög, Johanna L; Lacomble, Sylvain; Bouchet-Marquis, Cedric et al. (2016) 3D Architecture of the Trypanosoma brucei Flagella Connector, a Mobile Transmembrane Junction. PLoS Negl Trop Dis 10:e0004312
Park, J Genevieve; Palmer, Amy E (2015) Properties and use of genetically encoded FRET sensors for cytosolic and organellar Ca2+ measurements. Cold Spring Harb Protoc 2015:pdb.top066043
McCoy, Kelsey M; Tubman, Emily S; Claas, Allison et al. (2015) Physical limits on kinesin-5-mediated chromosome congression in the smallest mitotic spindles. Mol Biol Cell 26:3999-4014
Höög, Johanna L; Lötvall, Jan (2015) Diversity of extracellular vesicles in human ejaculates revealed by cryo-electron microscopy. J Extracell Vesicles 4:28680
Marc, Robert E; Anderson, James R; Jones, Bryan W et al. (2014) The AII amacrine cell connectome: a dense network hub. Front Neural Circuits 8:104
Weber, Britta; Tranfield, Erin M; Höög, Johanna L et al. (2014) Automated stitching of microtubule centerlines across serial electron tomograms. PLoS One 9:e113222

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