This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Plant Golgi stacks travel along actin filament bundles in a """"""""Stop and Go"""""""" pattern of movement. This Stop and Go pattern is characterized by alternations between rapid straight displacements (go phase), and random wiggling at set locations (stop phase). Wiggling Golgi stacks are associated transiently with ER export sites (tER) marked by the Sec13 protein, a component of the COPII vesicular transport machinery. This suggests that the ER-to-Golgi transport in plant cells takes place during transient interactions between these two compartments. We have investigated Golgi stacks and the ER in Arabidopsis thaliana and in alfalfa root meristem and columella cells, using electron tomography and immunoelectron microscopy. tER sites were identified by the presence of budding COPII vesicles labeled by antisera against AtSar1p. A fraction of the Golgi stacks were located close to ER export sites, and these Golgi appeared to be connected to the tER sites through a ribosome-excluding matrix. ER export sites tend to be organized around the cis-side of close Golgi stacks. All of the COPII vesicles were surrounded by a ribosome-excluding matrix, both while budding and during their transfer to the Golgi. COPII vesicles were seen within the Golgi matrix of docked Golgi stacks and these always displayed a small, irregularly shaped cis-most cisterna. Golgi stacks not connected to ER export sites contained few COPII vesicles in their matrices and lacked characteristic cis-cisternal assembly intermediates. In root columella cells, the ER is confined to cortical region of the cytoplasm. Golgi stacks in the cell cortex were also coupled to ER export sites via a matrix and displayed assembling cis-cisternae;those near the cell's center lacked these structures. Together, these findings demonstrate that COPII vesicle-mediated transport occurs during the """"""""stop"""""""" phase of Golgi movement, and that the process of stopping involves the formation of transient links between the ribosome-excluding matrix of forming COPII vesicles and the cis-side of the Golgi matrix.
| Giddings Jr, Thomas H; Morphew, Mary K; McIntosh, J Richard (2017) Preparing Fission Yeast for Electron Microscopy. Cold Spring Harb Protoc 2017: |
| Zhao, Xiaowei; Schwartz, Cindi L; Pierson, Jason et al. (2017) Three-Dimensional Structure of the Ultraoligotrophic Marine Bacterium ""Candidatus Pelagibacter ubique"". Appl Environ Microbiol 83: |
| Brown, Joanna R; Schwartz, Cindi L; Heumann, John M et al. (2016) A detailed look at the cytoskeletal architecture of the Giardia lamblia ventral disc. J Struct Biol 194:38-48 |
| Saheki, Yasunori; Bian, Xin; Schauder, Curtis M et al. (2016) Control of plasma membrane lipid homeostasis by the extended synaptotagmins. Nat Cell Biol 18:504-15 |
| Höög, Johanna L; Lacomble, Sylvain; Bouchet-Marquis, Cedric et al. (2016) 3D Architecture of the Trypanosoma brucei Flagella Connector, a Mobile Transmembrane Junction. PLoS Negl Trop Dis 10:e0004312 |
| Park, J Genevieve; Palmer, Amy E (2015) Properties and use of genetically encoded FRET sensors for cytosolic and organellar Ca2+ measurements. Cold Spring Harb Protoc 2015:pdb.top066043 |
| McCoy, Kelsey M; Tubman, Emily S; Claas, Allison et al. (2015) Physical limits on kinesin-5-mediated chromosome congression in the smallest mitotic spindles. Mol Biol Cell 26:3999-4014 |
| Höög, Johanna L; Lötvall, Jan (2015) Diversity of extracellular vesicles in human ejaculates revealed by cryo-electron microscopy. J Extracell Vesicles 4:28680 |
| Marc, Robert E; Anderson, James R; Jones, Bryan W et al. (2014) The AII amacrine cell connectome: a dense network hub. Front Neural Circuits 8:104 |
| Weber, Britta; Tranfield, Erin M; Höög, Johanna L et al. (2014) Automated stitching of microtubule centerlines across serial electron tomograms. PLoS One 9:e113222 |
Showing the most recent 10 out of 84 publications
