This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ape1 (Ape(delta)40) is a protein that participates in redox regulation of transcription factor function. The redox function of Ape1 involves reduction of oxidized cysteine residues within the DNA binding domains of several transcription factors, including c-Jun. E3330 is a small molecule containing a quinone. It binds directly to Ape1 and inhibits the redox function of Ape1. E3330 blocks the ability of Ape1 to reduce c-Jun. We wish to use PLIMSTEX (Protein-Ligand Interaction by MS, Titration and H/D exchange) to study the interactions of Ape1 to c-Jun or E3330. The method would enable us to determine the binding affinity constants between Ape1 and c-Jun or E3330 and perhaps to map the site of interaction of E3330 and Ape1.
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