This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.One of the core research efforts in the Michael Gross lab is the development of ionic liquids as matrices in MALDI. In this core/collaborative project, we wish to use ionic liquid matrices (ILMs) for phospholipids (PLs) because their use affords higher signal intensity, smaller spot size, improved spot homogeneity, better signal reproducibility, and comparable or better detection limits compared to that of the crystalline matrix 2,5-dihydroxybenzoic acid (2,5-DHB). The ionization products are comparable to those with 2,5-DHB although the use of ILMs gives a stronger tendency to produce alkali-metal-ion adducts and a lower extent of prompt fragmentation. An exciting opportunity in this area is the addition of the MALDI TOF/TOF instrument, which will enable research both in the ionization and the fragmentation of phospholipids.
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