This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.To use stable isotopically labeled precursors of pulmonary surfactant phospholipids to measure precursor utilization and surfactant turnover in premature infants who required mechanical ventilation at birth, 2 weeks, and >4 weeks of age. Study design: Infants of d28 weeks' gestation received simultaneous 24-hour intravenous infusions of [1,2,3,4- 13C4] palmitate and [1-13C1] acetate at birth, 2 weeks, and e4 weeks of life. Disaturated phospholipids were extracted from sequential tracheal aspirate samples obtained over a period of 2 weeks. Fractional catabolic rate (a measure of total turnover) and the fractional synthetic rates from plasma palmitate and de novo synthesis (acetate) were measured.The fractional catabolic rate increased from 25.3% 7.0% per day at birth to 53.8% 14.4% per day at 4 weeks (P = .001). The combined contribution from plasma palmitate and de novo synthesis to total synthesis increased from 44.2% 19.8% at birth to 85.2% 32.8% at 4 weeks (P = .03).
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