This proposal from the Massachusetts Institute of Technology requests our grant for a period of five years to support a Naional NMR Facility for Biomolecular Research located at the Francis Bitter National Magnet Laboratory. The overall mission of the Facility is to: (1) develop, operate and maintain five state-of-the-art superconducting NMR spectrometer systems; (2) to provide these advanced NMR facilities for collaborative and visitors' research projects; (3) to continue to carry out in-house basic and applied research to enhance the capabilities of the experimental NMR facilities and to delineate new biomedical applications; (4) to continue to disseminate to the scientific community the results of our research and to continue the on-going transfer of technology to industry; (5) to continue to train resident staff, collaborators and visitors in the application of new NMR methodology to the solution of biomedical problems. The complement of advanced instruments will allow users to pursue a broad program of research on NMR methodology, macromolecules and their complexes, cells, tissues, and small animals. It will be possible to probe all of the complexities of biological NMR in both the liquid and solid states. Proposed user activities in these areas are described in detail in this proposal. The major new technologies to be introduced are: (1) a high performance spectrometer of our own design and construction utilizing a 9.4 T/8.9 cm magnet for studies of metabolism in living systems; (2) a capability for performing photo-CIDNP studies on the high resolution 500 MHz spectrometer; (3) a unique high resolution, multinuclear, 600 MHz spectrometer employing a persistent mode 14 T/5 cm magnet of our own design and construction; (4) a distributed data processing network which will link the five operational spectrometers to a powerfully configured VAX-11/730 computer system.
Our aim i s to establish a comprehensive capability in high field analytical NMR for studying the structure and function of the diverse materials important in biological processes.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR000995-13
Application #
3103720
Study Section
(SSS)
Project Start
1976-05-01
Project End
1989-04-30
Budget Start
1988-05-01
Budget End
1989-04-30
Support Year
13
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Type
Organized Research Units
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Marintchev, Assen; Edmonds, Katherine A; Marintcheva, Boriana et al. (2009) Topology and regulation of the human eIF4A/4G/4H helicase complex in translation initiation. Cell 136:447-60
Frueh, Dominique P; Arthanari, Haribabu; Koglin, Alexander et al. (2009) A double TROSY hNCAnH experiment for efficient assignment of large and challenging proteins. J Am Chem Soc 131:12880-1
Frueh, Dominique P; Leed, Alison; Arthanari, Haribabu et al. (2009) Time-shared HSQC-NOESY for accurate distance constraints measured at high-field in (15)N-(13)C-ILV methyl labeled proteins. J Biomol NMR 45:311-8
Lentz, Margaret R; Westmoreland, Susan V; Lee, Vallent et al. (2008) Metabolic markers of neuronal injury correlate with SIV CNS disease severity and inoculum in the macaque model of neuroAIDS. Magn Reson Med 59:475-84
Hyberts, Sven G; Heffron, Gregory J; Tarragona, Nestor G et al. (2007) Ultrahigh-resolution (1)H-(13)C HSQC spectra of metabolite mixtures using nonlinear sampling and forward maximum entropy reconstruction. J Am Chem Soc 129:5108-16
Chen, Jingyang; Dupradeau, Francois-Yves; Case, David A et al. (2007) Nuclear magnetic resonance structural studies and molecular modeling of duplex DNA containing normal and 4'-oxidized abasic sites. Biochemistry 46:3096-107
Lentz, Margaret R; Kim, John P; Westmoreland, Susan V et al. (2005) Quantitative neuropathologic correlates of changes in ratio of N-acetylaspartate to creatine in macaque brain. Radiology 235:461-8
Kim, John P; Lentz, Margaret R; Westmoreland, Susan V et al. (2005) Relationships between astrogliosis and 1H MR spectroscopic measures of brain choline/creatine and myo-inositol/creatine in a primate model. AJNR Am J Neuroradiol 26:752-9
Peled, S; Cory, D G; Raymond, S A et al. (1999) Water diffusion, T(2), and compartmentation in frog sciatic nerve. Magn Reson Med 42:911-8
Mo, H; Dai, Y; Pochapsky, S S et al. (1999) 1H, 13C and 15N NMR assignments for a carbon monoxide generating metalloenzyme from Klebsiella pneumoniae. J Biomol NMR 14:287-8

Showing the most recent 10 out of 12 publications