Abstract

The bacterium Rhizobium meliloti produces exopolysaccharides, including succinoglycan and EPSII, which mediate invasion of alfalfa root nodules during establishment of symbiotic nitrogen fixation. The wild type, or normal forms, of these polysaccharides includemodifications, such as acetyl, succinyl, and pyruvyl groups. We have used genetic analyses to identify genes involved in adding particular groups to succinoglycan and have determined that mutations in two of these genes cause symbiotic defects. We have also determined that nutrients in bacterial growth media can determine the extent to which succinoglycan is modified by particular groups.
Our aim i s to determine how exopolysaccharides mediate nodule invasion, by generating more structural variants of these exopolysaccharides and comparing their biological activities in terms of mediating nodule invasion by the bacterium.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR000995-21
Application #
5221964
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
21
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Marintchev, Assen; Edmonds, Katherine A; Marintcheva, Boriana et al. (2009) Topology and regulation of the human eIF4A/4G/4H helicase complex in translation initiation. Cell 136:447-60
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Chen, Jingyang; Dupradeau, Francois-Yves; Case, David A et al. (2007) Nuclear magnetic resonance structural studies and molecular modeling of duplex DNA containing normal and 4'-oxidized abasic sites. Biochemistry 46:3096-107
Lentz, Margaret R; Kim, John P; Westmoreland, Susan V et al. (2005) Quantitative neuropathologic correlates of changes in ratio of N-acetylaspartate to creatine in macaque brain. Radiology 235:461-8
Kim, John P; Lentz, Margaret R; Westmoreland, Susan V et al. (2005) Relationships between astrogliosis and 1H MR spectroscopic measures of brain choline/creatine and myo-inositol/creatine in a primate model. AJNR Am J Neuroradiol 26:752-9
Peled, S; Cory, D G; Raymond, S A et al. (1999) Water diffusion, T(2), and compartmentation in frog sciatic nerve. Magn Reson Med 42:911-8
Mo, H; Dai, Y; Pochapsky, S S et al. (1999) 1H, 13C and 15N NMR assignments for a carbon monoxide generating metalloenzyme from Klebsiella pneumoniae. J Biomol NMR 14:287-8

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