L(24) is a membrane-spanning peptide of the structure KKL(24)KK and LA(12) has a structure KK(LA)(12)KK. We investigated effects of these peptides on alkyl chain order, alkyl chain motional freedom, polarity of the membrane interior, and on oxygen transport. As a model membrane we used liposomes and oriented membranes. We used PC-spin labels with nitroxide moiety located at the 5-, 7-, 10-, 12-, and 16- position. We showed that L(24) ordered the lipid bilayer, decreased motional freedom of alkyl chains and significantly increased the hydrophobicity of the membrane interior. The effects of LA(12) on these membrane properties were very weak.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001008-21
Application #
5222133
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
21
Fiscal Year
1996
Total Cost
Indirect Cost
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Sheng, Zhi-Guo; Huang, Wei; Liu, Yu-Xiang et al. (2013) Bisphenol A at a low concentration boosts mouse spermatogonial cell proliferation by inducing the G protein-coupled receptor 30 expression. Toxicol Appl Pharmacol 267:88-94
Liddle, Brendan J; Wanniarachchi, Sarath; Hewage, Jeewantha S et al. (2012) Electronic communication across diamagnetic metal bridges: a homoleptic gallium(III) complex of a redox-active diarylamido-based ligand and its oxidized derivatives. Inorg Chem 51:12720-8

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