We are using the Computer Graphics Laboratory resources to design and re-design a four helix bundle protein. The modest resolution crystal structure for this de novo designed four-helix bundle proto-type was recently published. Another crystal form was found which yielded a data set of higher resolution. We are solving this structure now. We will be using this unique structure to understan some of the protein design principles that were originally introduced. Further studies planned include using this new structural data to design mutations using the CGL graphics lab resources such as MidasPlus graphics software and gene manipulation software. The direction of the mutation currently focuses on understanding cooperativity in protein unfolding and what leads to fold specificity. Strictly structural studies will include testing whether this simple protein can be a scaffold for studying protein-protein interactions between loops of difficult to study proteins, such as membrane proteins with well-definited functional loops. Biochemical and structural studies will follow the new proteins designed using the CGL resources. The design will focus on adding function to understand the nature of the basic principles which allow proteins to carry minimal function.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001081-22
Application #
6119200
Study Section
Project Start
1999-07-01
Project End
2000-06-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
22
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Type
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143
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