High-grade squamous intraepithelial lesion (H-SIL) is an extremely frequent precancerous cervical lesion, leading to invasive cervical cancer if undetected and untreated. We are currently evaluating two new noninvasive methods for detection and treatment of H-SIL photodynamic therapy (PDT) and frequency domain photon migration (FDPM). PDT induces selective necrosis of dye sensitize malignant tissues when activated with light of appropriate wavelength. FDPM is a near-infrared spectroscopy technique for determining optical properties (i.e. absorption and scattering of light, ua and uUs) of tissues. Our objectives are 1) To determine the feasibility, safety, and efficacy of using PDT to treat H-SIL, 2) To quantitatively determine ua and uUs of normal cervical tissue and H-SIL in vivo, and to calculate physiologic parameters, specifically absolute concentration of oxy/deoxyhemoglobin and water in cervical tissue, 3) To assess the feasibility of differentiating normal cervical tissues from H-SIL based on the optical properties, 4) to monitor how cervical tissue optical and physiological properties change in response to PDT, 5) To optimize PDT light dosimetery and clinical outcome based on optical property measurements.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR001192-19
Application #
6280345
Study Section
Project Start
1998-05-15
Project End
1999-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
19
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of California Irvine
Department
Type
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697
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