This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We demonstrate the ability to store the spatial distribution of continuous wave laser beams focused at high numerical aperture within rubrene-doped polymer films. Scanning confocal fluorescent microscopy is subsequently used to image the microbeam distribution. Using the measured dose-response curve for the rubrene fluorescence intensity generated by the microbeam irradiation, we process confocal fluorescence images to provide spatial intensity distributions of the focused laser microbeam. These intensity distributions are fit to equations governing Gaussian beam propagation to quantify the microbeam propagation characteristics. The results demonstrate close agreement between the measured microbeam size and theoretical predictions. Practical applications of such photochromic polymer films in biomedical microscopy are suggested.
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