This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Red fluorescent protein (RFP) from Discosoma coral (drFP583) has recently received considerable attention due to its longer wavelength emission than the popular green fluorescent protein (GFP) from Aequorea victoria. Longer wavelength emitting proteins are of special interest in in vivo protein localization and gene expression studies, where interference from cell autofluorescence can be problematic. We would like to understand the chromophore maturation process and the structural requirements for red fluorescence. Mutants of RFP will be made to probe the structural mechanism of chromophore maturation and function.
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