This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Potassium channel domain structures: The first goal is to continue investigating the structural basis of the N-terminal cytoplasmic domain containing K channel tetramerization for modulating channel conductance. Our initial work of T1 domain is further expanded to the entire N-terminal domain of the channel and in complex with cytoplasmic regulatory proteins. Crystals of several domain variant and complexes with cytoplasmic protein components will be studied. The second goal is to obtain the high-resolution structure of bacterial KTN domain in complex with its cytoplasmic interacting protein. The structure will be used to understand the ligand-mediated gating mechanism of bacterial K transporters in general. Several ligand-protein complexes have been obtained for structural characterization.
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