This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Doniach and associates have used SSRL BL4-2 to extensively characterize denatured state sizes for a wide variety of proteins, but this will be the first, or one of the first, direct determinations of the denatured state sizes for a thermophilic-mesophilic protein pair using SAXS methods. These structural measurements will augment extensive thermodynamic measurements made on the denaturation of these proteins which suggest that the denatured states sizes should differ for the two proteins, even though they are similar in molecular weight. These structural data are the last measurements necessary to complete a publication on the comparative denaturation mechanisms of this thermophilic-mesophilic polymerase pair.
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