This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Four synthetic cholesteric lyquid-crystal polymers (CLCP)are able to bind nucleic acids in solution and behave as non-viral vectors, transfecting in cells. We expect to observe by SAXS the effect of enviromental changes (rapid dilution and pH-jumps)on the CLCPs structure, dispersed in water, chloroform and methanol. Finally we intend to understand the structural changes in the CLCP induced by commercial-DNA binding when dispersed in aqueous solution. Single crystals of CLCP already attained would permit us to obtain single crystal diffraction data in the low resolution range which would help to interpret the solution scattering curves. Rapid access is needed to untangle other experiments in course, presently confused.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001209-28
Application #
7598326
Study Section
Special Emphasis Panel (ZRG1-BPC-E (40))
Project Start
2007-03-01
Project End
2008-02-29
Budget Start
2007-03-01
Budget End
2008-02-29
Support Year
28
Fiscal Year
2007
Total Cost
$199
Indirect Cost
Name
Stanford University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
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