This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The goal of this project is to enable the simultaneous acquisition of x-ray diffraction data and single crystal absorption spectra in the UV/Visible and infrared regions. Analysis of the absorption spectrum in the UV (190 - 400 nm) and visible (400 - 800 nm) regions provides information about the electronic state of proteins while in the crystalline state. To explore this approach, a single crystal microspectrophotometer was set up on BL7-1 to record spectra from crystals on the goniometer. During diffraction experiments, spectroscopic measurements were analyzed to confirm the redox state. Data were collected from crystals containing mixtures of metal oxidation states. The resulting data allow the estimation of the mixed states. Based on these and previous experiments, the instrument is now being automated and incorporated into the beam line control system.
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