Ivem Installation

Mannella, Carmen A.

Abstract

Installation of the Resources IVEM was completed in May. The assembly of the microscope itself took two months, although the previous preparation of the lab took much longer. The anticontaminator was modified during installation to permit q60x tilt with all specimen rods to be used. The microscope consistantly performs extremely well. There has been no unplanned downtime since installation. The TEM resolution at 200 and 400kV is better than 0.3nm by optical diffraction. Gold lattice lines of 0.14nm can be seen at any time with a conventional specimen holder. The STEM point resolution is 1nm. The Gatan cryotransfer stage, purchased from this grant, can demonstrate 0.31nm carbon lattice lines at 60x tilt and -180xC. Magnification, exposure, and dose calibrations have been done. The TN-5500 X-ray microanalysis unit delivered with the IVEM has been repaired and now functions according to original specifications.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Center for Research Resources (NCRR)
• Type: Biotechnology Resource Grants (P41)
• Project #: 5P41RR001219-15
• Application #: 5222972
• Support Year: 15
• Fiscal Year: 1996

Publications

Booth, David M; Enyedi, Balázs; Geiszt, Miklós et al. (2016) Redox Nanodomains Are Induced by and Control Calcium Signaling at the ER-Mitochondrial Interface. Mol Cell 63:240-248

Mannella, Carmen A; Lederer, W Jonathan; Jafri, M Saleet (2013) The connection between inner membrane topology and mitochondrial function. J Mol Cell Cardiol 62:51-7

Takvorian, Peter M; Buttle, Karolyn F; Mankus, David et al. (2013) The multilayered interlaced network (MIN) in the sporoplasm of the microsporidium Anncaliia algerae is derived from Golgi. J Eukaryot Microbiol 60:166-78

Forbes, Stephen J; Martinelli, Daniel; Hsieh, Chyongere et al. (2012) Association of a protective monoclonal IgA with the O antigen of Salmonella enterica serovar Typhimurium impacts type 3 secretion and outer membrane integrity. Infect Immun 80:2454-63

Wang, Ruiwu; Zhong, Xiaowei; Meng, Xing et al. (2011) Localization of the dantrolene-binding sequence near the FK506-binding protein-binding site in the three-dimensional structure of the ryanodine receptor. J Biol Chem 286:12202-12

Marko, Michael; Leith, Ardean; Hsieh, Chyongere et al. (2011) Retrofit implementation of Zernike phase plate imaging for cryo-TEM. J Struct Biol 174:400-12

Springer, Deborah J; Ren, Ping; Raina, Ramesh et al. (2010) Extracellular fibrils of pathogenic yeast Cryptococcus gattii are important for ecological niche, murine virulence and human neutrophil interactions. PLoS One 5:e10978

Li, Chunhao; Sal, Melanie; Marko, Michael et al. (2010) Differential regulation of the multiple flagellins in spirochetes. J Bacteriol 192:2596-603

McEwen, Bruce F; Dong, Yimin (2010) Contrasting models for kinetochore microtubule attachment in mammalian cells. Cell Mol Life Sci 67:2163-72

Palladino, Michael J (2010) Modeling mitochondrial encephalomyopathy in Drosophila. Neurobiol Dis 40:40-5