Abstract

(Supported in part by CNRS grants to M. Bornens) This is part of an ongoing study of the structure of the human centrosome. Because centrosomes are extremely small (0.25 by 0.5 (m) and complex, the only way their morphology can be studied is with EM. Normally, centrosomes are examined in the EM after embedding and observation in conventional plastic sections. The resolution obtainable is somewhat limited using this technique. Our lab has isolated human centrosomes, and we have been studying them after negative staining. We have obtained high-resolution images showing the periodicity of the centriolar structure. To study the 3-D structure, we needed the IVEM automated tomography system because of the thickness of the whole-mount preparations and the dose-sensitivity of the negative-stain preparations. We also contemplate frozen-hydrated work in the future. On her visit Dr. Ruiz recorded six double-tilt series and one single-tilt tomographic series. A test reconstruction was made by Dr. Rath. This project will continue with the assistance of Dr. Radermacher.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001219-17
Application #
6280705
Study Section
Project Start
1998-01-01
Project End
1998-12-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
17
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Wadsworth Center
Department
Type
DUNS #
110521739
City
Menands
State
NY
Country
United States
Zip Code
12204

  Publications

Booth, David M; Enyedi, Balázs; Geiszt, Miklós et al. (2016) Redox Nanodomains Are Induced by and Control Calcium Signaling at the ER-Mitochondrial Interface. Mol Cell 63:240-248
Mannella, Carmen A; Lederer, W Jonathan; Jafri, M Saleet (2013) The connection between inner membrane topology and mitochondrial function. J Mol Cell Cardiol 62:51-7
Takvorian, Peter M; Buttle, Karolyn F; Mankus, David et al. (2013) The multilayered interlaced network (MIN) in the sporoplasm of the microsporidium Anncaliia algerae is derived from Golgi. J Eukaryot Microbiol 60:166-78
Forbes, Stephen J; Martinelli, Daniel; Hsieh, Chyongere et al. (2012) Association of a protective monoclonal IgA with the O antigen of Salmonella enterica serovar Typhimurium impacts type 3 secretion and outer membrane integrity. Infect Immun 80:2454-63
Wang, Ruiwu; Zhong, Xiaowei; Meng, Xing et al. (2011) Localization of the dantrolene-binding sequence near the FK506-binding protein-binding site in the three-dimensional structure of the ryanodine receptor. J Biol Chem 286:12202-12
Marko, Michael; Leith, Ardean; Hsieh, Chyongere et al. (2011) Retrofit implementation of Zernike phase plate imaging for cryo-TEM. J Struct Biol 174:400-12
Springer, Deborah J; Ren, Ping; Raina, Ramesh et al. (2010) Extracellular fibrils of pathogenic yeast Cryptococcus gattii are important for ecological niche, murine virulence and human neutrophil interactions. PLoS One 5:e10978
Li, Chunhao; Sal, Melanie; Marko, Michael et al. (2010) Differential regulation of the multiple flagellins in spirochetes. J Bacteriol 192:2596-603
McEwen, Bruce F; Dong, Yimin (2010) Contrasting models for kinetochore microtubule attachment in mammalian cells. Cell Mol Life Sci 67:2163-72
Palladino, Michael J (2010) Modeling mitochondrial encephalomyopathy in Drosophila. Neurobiol Dis 40:40-5

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