Abstract

(Supported in part by NIH GM 45983 and AHA 93-3030G to M. Lennartz). We have demonstrated that arachidonic acid (AA) is required for phagocytosis to occur and that an apparently unique (phagocytic ) phospholipase (pPL) is responsible for AA release. Inhibition of pPl results in the accumulaiton of electron lucent vesicles (ELV) in the cytosol of human monocytes. The ELV do not label with cationized ferritin, suggesting they are not contiguous with the plasma membrane. Our goal is to use the HVEM to better visualize the morphology of the ELV in 3-D, and to quantify the number of vesicles that contain an immunogold label when the cells are incubated with BSA. Many stereo HVEM and IVEM images were recorded from 0.5 and 1.0 (m thick sections. The data clearly reveal that vesicles in questions are no continuous with the plasma membrane and do not appear to be connected in a network arrangement. Lennartz, M.R., A.F.C. Yuen, S. M. Masi, D.G Russell, K.F. Buttle and J.J. Smith. (1997) Phospholipase A2 inhibition results in sequestration of plasma membrane into electron lucent vesicles during IgG-mediated phagocytosis. J. Cell Sci., 110:2041-2052. DISSEMINATION AND TRAINING SUBPROJECTS

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001219-17
Application #
6280720
Study Section
Project Start
1998-01-01
Project End
1998-12-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
17
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Wadsworth Center
Department
Type
DUNS #
110521739
City
Menands
State
NY
Country
United States
Zip Code
12204

  Publications

Booth, David M; Enyedi, Balázs; Geiszt, Miklós et al. (2016) Redox Nanodomains Are Induced by and Control Calcium Signaling at the ER-Mitochondrial Interface. Mol Cell 63:240-248
Mannella, Carmen A; Lederer, W Jonathan; Jafri, M Saleet (2013) The connection between inner membrane topology and mitochondrial function. J Mol Cell Cardiol 62:51-7
Takvorian, Peter M; Buttle, Karolyn F; Mankus, David et al. (2013) The multilayered interlaced network (MIN) in the sporoplasm of the microsporidium Anncaliia algerae is derived from Golgi. J Eukaryot Microbiol 60:166-78
Forbes, Stephen J; Martinelli, Daniel; Hsieh, Chyongere et al. (2012) Association of a protective monoclonal IgA with the O antigen of Salmonella enterica serovar Typhimurium impacts type 3 secretion and outer membrane integrity. Infect Immun 80:2454-63
Wang, Ruiwu; Zhong, Xiaowei; Meng, Xing et al. (2011) Localization of the dantrolene-binding sequence near the FK506-binding protein-binding site in the three-dimensional structure of the ryanodine receptor. J Biol Chem 286:12202-12
Marko, Michael; Leith, Ardean; Hsieh, Chyongere et al. (2011) Retrofit implementation of Zernike phase plate imaging for cryo-TEM. J Struct Biol 174:400-12
Springer, Deborah J; Ren, Ping; Raina, Ramesh et al. (2010) Extracellular fibrils of pathogenic yeast Cryptococcus gattii are important for ecological niche, murine virulence and human neutrophil interactions. PLoS One 5:e10978
Li, Chunhao; Sal, Melanie; Marko, Michael et al. (2010) Differential regulation of the multiple flagellins in spirochetes. J Bacteriol 192:2596-603
McEwen, Bruce F; Dong, Yimin (2010) Contrasting models for kinetochore microtubule attachment in mammalian cells. Cell Mol Life Sci 67:2163-72
Palladino, Michael J (2010) Modeling mitochondrial encephalomyopathy in Drosophila. Neurobiol Dis 40:40-5

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