Abstract

(Supported in part by NIH GM36806-12, NIH CA31760-1, 1P01 DE12328-1 to R. D. Goldman) We are attempting to determine the relationship between cytoskeletal intermediate filaments and the nuclear envelope/pore complex of keratinocytes. This is best carried out through the careful study of thick plastic-embedded sections prepared for HVEM. A single-axis tilt series tomographic reconstruction of a 0.25 (m plastic section from a normal keratinocyte was made and volume-rendered. The nuclear pores were clearly visible. Cells prepared with mild extraction were examined in 0.5(m thick sections on the HVEM. There appeared to be more cellular debris than expected for an extracted sample, and the nuclear pores were not easily identified. Correspondence and negatives were sent to Dr. Goldman for review, along with questions regarding another preparation with more extraction, and the possibility that the age of the cells is related to the sparse intermediate filaments.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001219-18
Application #
6119691
Study Section
Project Start
1999-01-01
Project End
1999-12-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
18
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Wadsworth Center
Department
Type
DUNS #
110521739
City
Menands
State
NY
Country
United States
Zip Code
12204

  Publications

Booth, David M; Enyedi, Balázs; Geiszt, Miklós et al. (2016) Redox Nanodomains Are Induced by and Control Calcium Signaling at the ER-Mitochondrial Interface. Mol Cell 63:240-248
Mannella, Carmen A; Lederer, W Jonathan; Jafri, M Saleet (2013) The connection between inner membrane topology and mitochondrial function. J Mol Cell Cardiol 62:51-7
Takvorian, Peter M; Buttle, Karolyn F; Mankus, David et al. (2013) The multilayered interlaced network (MIN) in the sporoplasm of the microsporidium Anncaliia algerae is derived from Golgi. J Eukaryot Microbiol 60:166-78
Forbes, Stephen J; Martinelli, Daniel; Hsieh, Chyongere et al. (2012) Association of a protective monoclonal IgA with the O antigen of Salmonella enterica serovar Typhimurium impacts type 3 secretion and outer membrane integrity. Infect Immun 80:2454-63
Wang, Ruiwu; Zhong, Xiaowei; Meng, Xing et al. (2011) Localization of the dantrolene-binding sequence near the FK506-binding protein-binding site in the three-dimensional structure of the ryanodine receptor. J Biol Chem 286:12202-12
Marko, Michael; Leith, Ardean; Hsieh, Chyongere et al. (2011) Retrofit implementation of Zernike phase plate imaging for cryo-TEM. J Struct Biol 174:400-12
Springer, Deborah J; Ren, Ping; Raina, Ramesh et al. (2010) Extracellular fibrils of pathogenic yeast Cryptococcus gattii are important for ecological niche, murine virulence and human neutrophil interactions. PLoS One 5:e10978
Li, Chunhao; Sal, Melanie; Marko, Michael et al. (2010) Differential regulation of the multiple flagellins in spirochetes. J Bacteriol 192:2596-603
McEwen, Bruce F; Dong, Yimin (2010) Contrasting models for kinetochore microtubule attachment in mammalian cells. Cell Mol Life Sci 67:2163-72
Palladino, Michael J (2010) Modeling mitochondrial encephalomyopathy in Drosophila. Neurobiol Dis 40:40-5

Showing the most recent 10 out of 252 publications