This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ABSTRACT: SPIDER is an extensive image processing software package specifically designed for 3D reconstruction. Web is the graphics interface for displaying image files created in SPIDER. SPIDER has been evolving in Dr. Frank?s Lab since the late 1970?s and contains appropriate tools from single particle reconstruction, classification analysis, and electron tomography. SPIDER is continually expanded, upgraded and maintained. It is available to outside uses at a nominal cost and is currently licensed in 144 laboratories worldwide. The web site is: www.wadsworth.org/sp
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