Abstract

This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.ABSTRACT: Karolyn Buttle is supported by the grant, in part, to train users on the HVEM. She is supervised by M. Marko, and is kept abreast of the latest technical information on the HVEM operation by D. Barnard. During HVEM training sessions. K. Buttle sits with the user, and gives instructions on how to tilt the specimen, use the TV system for focusing, take pictures, etc. She is then responsible for assisting the user should any problems arise, and for changing specimens and developing negatives. In collaboration with M. Marko, she helps the user decide the best thickness and stain to use for the project, and gives advice on further work that might involve use of Sterecon or tomography. In many cases, K. Buttle also trains the user in thick-section techniques, and in how to stain sections for HVEM. In addition to her teaching/training responsibilities, K. Buttle is also responsible for HVEM user correspondence and for our mailed in grid screening service.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001219-26
Application #
7598361
Study Section
Special Emphasis Panel (ZRG1-BST-D (40))
Project Start
2007-02-01
Project End
2008-01-31
Budget Start
2007-02-01
Budget End
2008-01-31
Support Year
26
Fiscal Year
2007
Total Cost
$2,291
Indirect Cost

  Institution

Name
Wadsworth Center
Department
Type
DUNS #
153695478
City
Menands
State
NY
Country
United States
Zip Code
12204

  Publications

Booth, David M; Enyedi, Balázs; Geiszt, Miklós et al. (2016) Redox Nanodomains Are Induced by and Control Calcium Signaling at the ER-Mitochondrial Interface. Mol Cell 63:240-248
Mannella, Carmen A; Lederer, W Jonathan; Jafri, M Saleet (2013) The connection between inner membrane topology and mitochondrial function. J Mol Cell Cardiol 62:51-7
Takvorian, Peter M; Buttle, Karolyn F; Mankus, David et al. (2013) The multilayered interlaced network (MIN) in the sporoplasm of the microsporidium Anncaliia algerae is derived from Golgi. J Eukaryot Microbiol 60:166-78
Forbes, Stephen J; Martinelli, Daniel; Hsieh, Chyongere et al. (2012) Association of a protective monoclonal IgA with the O antigen of Salmonella enterica serovar Typhimurium impacts type 3 secretion and outer membrane integrity. Infect Immun 80:2454-63
Wang, Ruiwu; Zhong, Xiaowei; Meng, Xing et al. (2011) Localization of the dantrolene-binding sequence near the FK506-binding protein-binding site in the three-dimensional structure of the ryanodine receptor. J Biol Chem 286:12202-12
Marko, Michael; Leith, Ardean; Hsieh, Chyongere et al. (2011) Retrofit implementation of Zernike phase plate imaging for cryo-TEM. J Struct Biol 174:400-12
Springer, Deborah J; Ren, Ping; Raina, Ramesh et al. (2010) Extracellular fibrils of pathogenic yeast Cryptococcus gattii are important for ecological niche, murine virulence and human neutrophil interactions. PLoS One 5:e10978
Li, Chunhao; Sal, Melanie; Marko, Michael et al. (2010) Differential regulation of the multiple flagellins in spirochetes. J Bacteriol 192:2596-603
McEwen, Bruce F; Dong, Yimin (2010) Contrasting models for kinetochore microtubule attachment in mammalian cells. Cell Mol Life Sci 67:2163-72
Palladino, Michael J (2010) Modeling mitochondrial encephalomyopathy in Drosophila. Neurobiol Dis 40:40-5

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