Our experimental approach utilizes x-ray CT-derived indicator dilution curves. Two projects were completed this part year. Project I: This study evaluates how well the hemodynamic behavior of recruitable and non-recruitable components of the myocardial microvasculature can be quantitated in the intact heart with hole-body CT anesthetized pigs. Sequential injections of a suspension of nonradioactive, 15mm diameter, microspheres into a selected coronary artery. Regional myocardial perfusion (F, ml/g/ min), intramyocardial blood volume (r, ml/g) were estimated from these dynamic CT image sequences. For the intramyocardial microcirculation r=AoF+BoF1/2, where A=0.016 and B=0.076, the local slope of the relationship is an index of the transit time through the intramyocardiaal blood volume. The significance of this study is that the blood volume-to-flow relationship may be quantitative indicator of intramyocardial microvascular functional status. Project II: Anesthetized pigs scans were performed before and during occlusion and at 30 minutes time intervals during reperfusion of a coronary artery that has been occluded for either 0, 15, 30, 60, or 120 minutes. Regional myocardial perfusion (F), intramyocardial blood volume (r) and myocardial microcirculatory permeability-surface area product (PS, ml/g/min) were estimated from these dynamic CT images sequences. With increasing duration of ischemia the PS product remained unchanged (p>.1), but an index of Permeability (PS/r) increased with progressive duration of ischemia (p<.01). The significance of this study is that these studies are supporting the hypothesis that CT-based estimates of microvascular permeability provide a means for detecting for potential ischemia in adequately perfused myocardium.
| Bassingthwaighte, James B; Butterworth, Erik; Jardine, Bartholomew et al. (2012) Compartmental modeling in the analysis of biological systems. Methods Mol Biol 929:391-438 |
| Dash, Ranjan K; Bassingthwaighte, James B (2010) Erratum to: Blood HbO2 and HbCO2 dissociation curves at varied O2, CO2, pH, 2,3-DPG and temperature levels. Ann Biomed Eng 38:1683-701 |
| Bassingthwaighte, James B; Raymond, Gary M; Butterworth, Erik et al. (2010) Multiscale modeling of metabolism, flows, and exchanges in heterogeneous organs. Ann N Y Acad Sci 1188:111-20 |
| Dash, Ranjan K; Bassingthwaighte, James B (2006) Simultaneous blood-tissue exchange of oxygen, carbon dioxide, bicarbonate, and hydrogen ion. Ann Biomed Eng 34:1129-48 |
| Dash, Ranjan K; Bassingthwaighte, James B (2004) Blood HbO2 and HbCO2 dissociation curves at varied O2, CO2, pH, 2,3-DPG and temperature levels. Ann Biomed Eng 32:1676-93 |
| Kellen, Michael R; Bassingthwaighte, James B (2003) Transient transcapillary exchange of water driven by osmotic forces in the heart. Am J Physiol Heart Circ Physiol 285:H1317-31 |
| Kellen, Michael R; Bassingthwaighte, James B (2003) An integrative model of coupled water and solute exchange in the heart. Am J Physiol Heart Circ Physiol 285:H1303-16 |
| Wang, C Y; Bassingthwaighte, J B (2001) Capillary supply regions. Math Biosci 173:103-14 |
| Swanson, K R; True, L D; Lin, D W et al. (2001) A quantitative model for the dynamics of serum prostate-specific antigen as a marker for cancerous growth: an explanation for a medical anomaly. Am J Pathol 158:2195-9 |
| Swanson, K R; Alvord Jr, E C; Murray, J D (2000) A quantitative model for differential motility of gliomas in grey and white matter. Cell Prolif 33:317-29 |
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