The objective is to generate a nonfouling surface that is patterned with defined patches of cell surface receptor ligands. A cell culture system will then be used to identify the ligands that are capable of keeping vascular smooth muscles cells in a quiescent, non-proliferative phenotype. The geometry of the patches can influence the shape and the size of the cells, which is hypothesized to be another important factor influencing the physiological state of the cells. The ligands are bound to the surface by means of a fluorocarbon-PEG-peptide amphiphile. The hydrophobic tail of the surfactant strongly adsorbs to a fluorinated surface and functions as an anchoring molecule for the ligand.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001296-16
Application #
6345043
Study Section
Project Start
2000-09-01
Project End
2001-08-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
16
Fiscal Year
2000
Total Cost
$8,590
Indirect Cost
Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
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