This study aims to determine whether human monocytes/macrophages cultured on pyrolytic carbon (PYC) can oxidize the surface. Oxidation of the pyrolitic carbon is achieved by a number of methods, and surface analytical methods are employed to compare the surfaces so obtained to those observed after cell adhesion studies have been performed. SIMS was employed to characterize the prepared surface as well as to determine how successful various cleaning strategies are in removing adherent proteins and cells, which is necessary for subsequent sample analysis.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001296-17
Application #
6492614
Study Section
Project Start
2001-09-01
Project End
2002-08-31
Budget Start
Budget End
Support Year
17
Fiscal Year
2001
Total Cost
Indirect Cost
Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
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