The specific aim of this project is to measure calcium flux in single cells by phase-sensitive flow cytometry using calcium indicator dyes, such as, Indo-1 and Calcium Green. At present, calcium flux measurements are made using both single and dual-laser excitation coupled with ratiometric fluorescence detection methods employing optical bandpass filters and analog ratioing electronics. We will explore the utilization of differences in lifetimes between bound and unbound calcium probes to resolve fluorescence emission signals and thus eliminate the need for ratiometric measurements to get the concentration of a cellular constituent.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001315-14
Application #
5223171
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
14
Fiscal Year
1996
Total Cost
Indirect Cost
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