In cooperation with the National Flow Cytometry Resource, Los Alamos National Laboratory, Los Alamos, NM, USA, systematic investigations have been carried out using a well-established and -characterized two-step carcinogenesis model in 2-D and 3-D culture. Flow cytometric analysis of rhodamine 123 and 10N-nonyl-acridine orange staining simultaneously with the DNA stain Hoechst 33342 allowed for the determination of mitochondrial mass, activity, and function in viable, differently transformed cells as a function of location in 3-D cultures and relative to each other. A multiparameter/ multilaser flow cytometer has been used for data accquisition and the IDLYC program developed by the National Flow Cytometry Resource has been applied for data analysis. Some results have already been published: L.A. Kunz-Schughart, R.C. Habbersett, J.P. Freyer. Mitochondrial function in oncogene-transfected rat fibroblasts isolated from multicellular spheroids. Am. J. Physiol. 273: C1487-C1495, 1997. An additional manuscript in preparation will be submitted in the near future: L.A. Kunz-Schughart, R.C. Habbersett, J.P. Freyer. Impact of proliferative activity and tumorigenic conversion on mitochondrial function of fibroblasts in 2-D and 3-D culture.
| Frumkin, Jesse P; Patra, Biranchi N; Sevold, Anthony et al. (2016) The interplay between chromosome stability and cell cycle control explored through gene-gene interaction and computational simulation. Nucleic Acids Res 44:8073-85 |
| Johnson, Leah M; Gao, Lu; Shields IV, C Wyatt et al. (2013) Elastomeric microparticles for acoustic mediated bioseparations. J Nanobiotechnology 11:22 |
| Micheva-Viteva, Sofiya N; Shou, Yulin; Nowak-Lovato, Kristy L et al. (2013) c-KIT signaling is targeted by pathogenic Yersinia to suppress the host immune response. BMC Microbiol 13:249 |
| Ai, Ye; Sanders, Claire K; Marrone, Babetta L (2013) Separation of Escherichia coli bacteria from peripheral blood mononuclear cells using standing surface acoustic waves. Anal Chem 85:9126-34 |
| Sanders, Claire K; Mourant, Judith R (2013) Advantages of full spectrum flow cytometry. J Biomed Opt 18:037004 |
| Cushing, Kevin W; Piyasena, Menake E; Carroll, Nick J et al. (2013) Elastomeric negative acoustic contrast particles for affinity capture assays. Anal Chem 85:2208-15 |
| Houston, Jessica P; Naivar, Mark A; Jenkins, Patrick et al. (2012) Capture of Fluorescence Decay Times by Flow Cytometry. Curr Protoc Cytom 59:1.25.1-1.25.21 |
| Marina, Oana C; Sanders, Claire K; Mourant, Judith R (2012) Effects of acetic acid on light scattering from cells. J Biomed Opt 17:085002-1 |
| Chen, Jun; Carter, Mark B; Edwards, Bruce S et al. (2012) High throughput flow cytometry based yeast two-hybrid array approach for large-scale analysis of protein-protein interactions. Cytometry A 81:90-8 |
| Piyasena, Menake E; Austin Suthanthiraraj, Pearlson P; Applegate Jr, Robert W et al. (2012) Multinode acoustic focusing for parallel flow cytometry. Anal Chem 84:1831-9 |
Showing the most recent 10 out of 240 publications
