Signaling through multisubunit immune receptors is regulated through intracellular tyrosine kinases. These pathways require macromolecular assemblies involving intracellular tyrosine activation motifs (ITAMs) on the receptors and kinases such as syk and fgr. This project aims at examining the order of assembly, the affinities and kinetics of the molecular steps in cell activation. A complete description is provided in the collaborative projects of the Year 16-20 NFCR renewal. In brief, ITAM or kinase reagents are being displayed on beads through avidin-biotin or GS-GST linkages. The second components are fluorescently labeled and conventional as well as rapid mix flow cytometry will be used to detect and analyze the binding interactions. We have succeeded in displaying phosphoryated ITAMs on beads and detecting them with anti-phosphotyrosine antibodies.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
3P41RR001315-16
Application #
6280962
Study Section
Project Start
1998-07-01
Project End
1999-06-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
16
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Los Alamos National Lab
Department
Type
DUNS #
City
Los Alamos
State
NM
Country
United States
Zip Code
87545
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