We investigated the time dependent effects of 8 Gy of gamma radiation on cyclin-dependent kinases (Cdks) and incorporation of BrdU through the S phase of Chinese hamster ovary (CHO) cells. Results obtained indicated that the transient G1-phase delay or G1/S phase check point is mediated by inhibition of cyclin E-Cdk2 and point to the existence of a radiation induced S phase checkpoint located about 75% into S phase involving the inhibition of cyclin A-Cdk2 by a p53/Waf1-independent pathway in CHO cells. Currently we are also investigating the role of cell cycle regulatory proteins, cyclin D1 and p27, in DNA damage check point regulation. Levels of these proteins have been found to be reduced in irradiated normal, human cells.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001315-18
Application #
6327954
Study Section
Project Start
2000-07-01
Project End
2001-06-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
18
Fiscal Year
2000
Total Cost
$7,662
Indirect Cost
Name
Los Alamos National Lab
Department
Type
DUNS #
City
Los Alamos
State
NM
Country
United States
Zip Code
87545
Frumkin, Jesse P; Patra, Biranchi N; Sevold, Anthony et al. (2016) The interplay between chromosome stability and cell cycle control explored through gene-gene interaction and computational simulation. Nucleic Acids Res 44:8073-85
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