THIS SUBPROPOSAL CONSTITUTES A PILOT PROJECT EXAMINING THE EVENTS UNDERLYING GROSS TISSUE DEGENERATION. THE MODEL SYSTEM IS HOLOTHUROIDEAN ISOSTICHOPUS BADIONOTUS. THIS ANIMAL EXHIBITS EXTREME PRESSURE DEPENDENT LOCAL DEGENERATION OF THE BODY WALL. A MAJOR COMPONENT OF THE BODY WALL IS A THICK CONNECTIVE TISSUE LAYER. THE MECHANISM IS UNCLEAR BUT MAY INVOLVE CALCIUM DEPENDENT CELL DEATH AND ENZYME ACTIVATION. INITIAL STUDIES SHOW A MARKED AND TRANSIENT INFLUX OF CALCIUM DURING THE FIRST STAGES OF DEGENERATION SUGGESTING THE POSSIBILITY OF CALCIUM MEDIATED EVENTS. THESE EVENTS, BOTH FLUX AND DEGENERATION, ARE OBVIATED BY THE ADDITION OF ANTIOXIDANTS SUGGESTING A ROLE OF FREE RADICALS.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
3P41RR001395-14S1
Application #
5223344
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
14
Fiscal Year
1996
Total Cost
Indirect Cost
Demidenko, Eugene; Glaholt, S P; Kyker-Snowman, E et al. (2017) Single toxin dose-response models revisited. Toxicol Appl Pharmacol 314:12-23
Chowanadisai, Winyoo; Messerli, Shanta M; Miller, Daniel H et al. (2016) Cisplatin Resistant Spheroids Model Clinically Relevant Survival Mechanisms in Ovarian Tumors. PLoS One 11:e0151089
De Martino, Federico; Moerel, Michelle; Ugurbil, Kamil et al. (2015) Less noise, more activation: Multiband acquisition schemes for auditory functional MRI. Magn Reson Med 74:462-7
Brodsky, Alexander S; Fischer, Andrew; Miller, Daniel H et al. (2014) Expression profiling of primary and metastatic ovarian tumors reveals differences indicative of aggressive disease. PLoS One 9:e94476
Van Mooy, Benjamin A S; Hmelo, Laura R; Fredricks, Helen F et al. (2014) Quantitative exploration of the contribution of settlement, growth, dispersal and grazing to the accumulation of natural marine biofilms on antifouling and fouling-release coatings. Biofouling 30:223-36
De Martino, Federico; Zimmermann, Jan; Muckli, Lars et al. (2013) Cortical depth dependent functional responses in humans at 7T: improved specificity with 3D GRASE. PLoS One 8:e60514
De Martino, Federico; Moerel, Michelle; van de Moortele, Pierre-Francois et al. (2013) Spatial organization of frequency preference and selectivity in the human inferior colliculus. Nat Commun 4:1386
Vang, Souriya; Wu, Hsin-Ta; Fischer, Andrew et al. (2013) Identification of ovarian cancer metastatic miRNAs. PLoS One 8:e58226
Chowanadisai, Winyoo; Graham, David M; Keen, Carl L et al. (2013) Neurulation and neurite extension require the zinc transporter ZIP12 (slc39a12). Proc Natl Acad Sci U S A 110:9903-8
Graham, David M; Messerli, Mark A; Pethig, Ronald (2012) Spatial manipulation of cells and organelles using single electrode dielectrophoresis. Biotechniques 52:39-43

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