This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is part of an ongoing effort to integrate our electrochemical sensor technologies with opportunitites in chemical sensing by photon imaging. To do this we have constructed a photon counting station for use as an illuminometer and collection of fluorescence signals through a pulled fiber optic. A Hamamatsu R464 photo-multiplier tube is used for photon detection. A National instruments PCI-6036E is used to count the events and control a uniblitz vs-25 shutter. The software interface has been constructed using Lab-View 7. The Uniblitz shutter drive utilizes a Microchip PIC16F628 micro-controller pulse width modulator to drive a boost converter, converting 5 volts to 70 volts with a single 22uh inductor. The Uniblitz driver circuit serves as the basis for a precision circuit for piezo tube control. The Pic16F628 also has on the chip a 16 bit counter, we plan to use this to eliminate the PCI-6036e allowing the device to operate from a single RS232 port. With this photon counting system we can explore the use of luminescent chemical sensors and weak florescent signals located in cells and in the boundary layers

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001395-24
Application #
7357333
Study Section
Special Emphasis Panel (ZRG1-BPC-H (40))
Project Start
2005-12-01
Project End
2006-11-30
Budget Start
2005-12-01
Budget End
2006-11-30
Support Year
24
Fiscal Year
2006
Total Cost
$12,252
Indirect Cost
Name
Marine Biological Laboratory
Department
Type
DUNS #
001933779
City
Woods Hole
State
MA
Country
United States
Zip Code
02543
Demidenko, Eugene; Glaholt, S P; Kyker-Snowman, E et al. (2017) Single toxin dose-response models revisited. Toxicol Appl Pharmacol 314:12-23
Chowanadisai, Winyoo; Messerli, Shanta M; Miller, Daniel H et al. (2016) Cisplatin Resistant Spheroids Model Clinically Relevant Survival Mechanisms in Ovarian Tumors. PLoS One 11:e0151089
De Martino, Federico; Moerel, Michelle; Ugurbil, Kamil et al. (2015) Less noise, more activation: Multiband acquisition schemes for auditory functional MRI. Magn Reson Med 74:462-7
Van Mooy, Benjamin A S; Hmelo, Laura R; Fredricks, Helen F et al. (2014) Quantitative exploration of the contribution of settlement, growth, dispersal and grazing to the accumulation of natural marine biofilms on antifouling and fouling-release coatings. Biofouling 30:223-36
Brodsky, Alexander S; Fischer, Andrew; Miller, Daniel H et al. (2014) Expression profiling of primary and metastatic ovarian tumors reveals differences indicative of aggressive disease. PLoS One 9:e94476
De Martino, Federico; Zimmermann, Jan; Muckli, Lars et al. (2013) Cortical depth dependent functional responses in humans at 7T: improved specificity with 3D GRASE. PLoS One 8:e60514
De Martino, Federico; Moerel, Michelle; van de Moortele, Pierre-Francois et al. (2013) Spatial organization of frequency preference and selectivity in the human inferior colliculus. Nat Commun 4:1386
Vang, Souriya; Wu, Hsin-Ta; Fischer, Andrew et al. (2013) Identification of ovarian cancer metastatic miRNAs. PLoS One 8:e58226
Chowanadisai, Winyoo; Graham, David M; Keen, Carl L et al. (2013) Neurulation and neurite extension require the zinc transporter ZIP12 (slc39a12). Proc Natl Acad Sci U S A 110:9903-8
Olman, Cheryl A; Harel, Noam; Feinberg, David A et al. (2012) Layer-specific fMRI reflects different neuronal computations at different depths in human V1. PLoS One 7:e32536

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