This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Animals vary dramatically in their ability to regenerate lost body parts. Many cnidarians (e.g., anemones), annelids (segmented worms), and platyhelminths (flatworms), for example, have fantastic regeneration abilities and are capable of regenerating an entire adult from a small portion of the original animal. In contrast, most amniote vertebrates (mammals, birds, reptiles) and ecdysozoan phyla (e.g., arthropods, nematodes) are relatively poor regenerators; though capable of some limited regeneration, almost none of these organisms can regenerate parts of their primary body axis and none can regenerate a complete body from a small fragment. The phylogenetic distribution of regeneration potential across animals strongly suggests that the ancestral animal had extensive regeneration abilities and that loss of regeneration abilities has been a major evolutionary trend in many animal lineages. Understanding the ultimate and proximate causes of regeneration loss requires a detailed understanding of the evolutionary forces and developmental mechanisms operating in regenerating and non-regenerating species, preferably closely related ones. We have identified a group of small, freshwater annelid (segmented) worms, the naidine oligochaetes, that includes closely related species differing markedly in their ability to regenerate their heads. Head regeneration appears to be ancestral for the group and non-anteriorly regenerating species are likely to represent at least two independent and relatively recent evolutionary losses of regeneration ability. Naidines are therefore an excellent system in which to investigate the underlying causes of natural losses of regeneration ability. We are currently investigating a range of developmental phenomena (cell division, programmed cell death, cell migration, nervous system dynamics, body patterning) across both regenerating and non-regenerating species (NSF grant IOB-0520389) in order to identify processes which occur normally and which fail to occur in non-regenerating species. We are now eager to expand our studies to include bioelectric events, such as ion currents and voltage gradients, which are increasingly being recognized as intimately linked to the potential to regenerate. Our main goal for the current project is to describe membrane voltage changes and ion currents during the first 24 hours following head amputation in at least one regenerating species (our primary focus would be on Pristina leidyi, which is particularly transparent and easy to manipulate) and then to investigate these features in at least one of the non-regenerating species. Our expectation is that regenerating and non-regenerating species will display markedly different patterns of post-amputation voltage changes and ion fluxes.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001395-25
Application #
7598521
Study Section
Special Emphasis Panel (ZRG1-BPC-H (40))
Project Start
2006-12-01
Project End
2007-11-30
Budget Start
2006-12-01
Budget End
2007-11-30
Support Year
25
Fiscal Year
2007
Total Cost
$1,173
Indirect Cost
Name
Marine Biological Laboratory
Department
Type
DUNS #
001933779
City
Woods Hole
State
MA
Country
United States
Zip Code
02543
Demidenko, Eugene; Glaholt, S P; Kyker-Snowman, E et al. (2017) Single toxin dose-response models revisited. Toxicol Appl Pharmacol 314:12-23
Chowanadisai, Winyoo; Messerli, Shanta M; Miller, Daniel H et al. (2016) Cisplatin Resistant Spheroids Model Clinically Relevant Survival Mechanisms in Ovarian Tumors. PLoS One 11:e0151089
De Martino, Federico; Moerel, Michelle; Ugurbil, Kamil et al. (2015) Less noise, more activation: Multiband acquisition schemes for auditory functional MRI. Magn Reson Med 74:462-7
Van Mooy, Benjamin A S; Hmelo, Laura R; Fredricks, Helen F et al. (2014) Quantitative exploration of the contribution of settlement, growth, dispersal and grazing to the accumulation of natural marine biofilms on antifouling and fouling-release coatings. Biofouling 30:223-36
Brodsky, Alexander S; Fischer, Andrew; Miller, Daniel H et al. (2014) Expression profiling of primary and metastatic ovarian tumors reveals differences indicative of aggressive disease. PLoS One 9:e94476
De Martino, Federico; Zimmermann, Jan; Muckli, Lars et al. (2013) Cortical depth dependent functional responses in humans at 7T: improved specificity with 3D GRASE. PLoS One 8:e60514
De Martino, Federico; Moerel, Michelle; van de Moortele, Pierre-Francois et al. (2013) Spatial organization of frequency preference and selectivity in the human inferior colliculus. Nat Commun 4:1386
Vang, Souriya; Wu, Hsin-Ta; Fischer, Andrew et al. (2013) Identification of ovarian cancer metastatic miRNAs. PLoS One 8:e58226
Chowanadisai, Winyoo; Graham, David M; Keen, Carl L et al. (2013) Neurulation and neurite extension require the zinc transporter ZIP12 (slc39a12). Proc Natl Acad Sci U S A 110:9903-8
Graham, David M; Messerli, Mark A; Pethig, Ronald (2012) Spatial manipulation of cells and organelles using single electrode dielectrophoresis. Biotechniques 52:39-43

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