Abstract

Among the copper-dependent amine oxidases the characterization of the active site cofactor in lysyl oxidase is of particular interest, due to the biological importance of this enzyme in connective tissue proteins. It initiates the covalent cross-linking in collagen and elastin by oxidative deamination of peptidyl lysine or hydroxylysine. Studies have shown that abnormal expression of lysyl oxidase is associated with several pathologic conditions. Severe defects in connective tissues are associated with a deficiency of lysyl oxidase. Recently, lysyl oxidase was purified from calf aorta and was derivatized by 14C-phenylhydrazine. Proteolysis of derivated protein and characterization of labeled peptides by mass spectral and sequencing techniques will be used to obtain definitive structural proof regarding the nature of the active site cofactor and its mode of peptide attachment in bovine aorta lysyl oxidase. The use of mass spectrometry is critical in characterization of the novel linkages of the organic cofactor to the peptide backbone.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR001614-15
Application #
5223380
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
15
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

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Bikle, Daniel D (2016) Extraskeletal actions of vitamin D. Ann N Y Acad Sci 1376:29-52
Twiss, Jeffery L; Fainzilber, Mike (2016) Neuroproteomics: How Many Angels can be Identified in an Extract from the Head of a Pin? Mol Cell Proteomics 15:341-3
Cil, Onur; Phuan, Puay-Wah; Lee, Sujin et al. (2016) CFTR activator increases intestinal fluid secretion and normalizes stool output in a mouse model of constipation. Cell Mol Gastroenterol Hepatol 2:317-327
Posch, Christian; Sanlorenzo, Martina; Vujic, Igor et al. (2016) Phosphoproteomic Analyses of NRAS(G12) and NRAS(Q61) Mutant Melanocytes Reveal Increased CK2? Kinase Levels in NRAS(Q61) Mutant Cells. J Invest Dermatol 136:2041-2048
Julien, Olivier; Zhuang, Min; Wiita, Arun P et al. (2016) Quantitative MS-based enzymology of caspases reveals distinct protein substrate specificities, hierarchies, and cellular roles. Proc Natl Acad Sci U S A 113:E2001-10

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