Training and Dissemination are essential functions of the Resource. Only with appropriate training can users make productive, efficient and safe use of the Resource facilities. The training and dissemination in the last year has primarily involved the following: TRAINING 1) Teaching users in safe and efficient use of the X-9B beamline for XAS and diffraction, including entirely new user groups. 2) Teaching users in techniques of x-ray crystallography data collection, which is a new activity for the Resource. 3) Teaching users in techniques of infrared spectroscopy to allow their use of the U4-IR beamline facility. 4) Teaching users in techniques of X-ray footprinting to allow their use of the X-19 C footprinting beamline. 5) The training consists of hands on instruction and preparation of user manuals. DISSEMINATION 1) A newsletter, """"""""Proteins in Synch"""""""" is regularly published notifying users of core research improvements and beamline upgrades. A copy of the most recent issue is included in the appendix. The newsletter generally appears two times per year. 2) Dr. Chance and Dr. Brenowitz have given a number of invited talks over the past year discussing XAS data analysis and footprinting techniques respectively. Also, Ms. Sclavi (Graduate student) and Dr. Scheuring (Post-Doc) have presented Resource results at a number of meetings like, the Biophysical Society, the NSLS annual meeting, The International Biophysics Congress, The International XAS conference, and the RNA society meeting. 3) The numerous core, collaborative, and service publications (papers and published abstracts) are an important aspect of resource dissemination, and are listed in the report.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001633-14
Application #
5223462
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
14
Fiscal Year
1996
Total Cost
Indirect Cost
Vongsvivut, Jitraporn; Fernandez, Jason; Ekgasit, Sanong et al. (2004) Characterization of supported cylinder-planar germanium waveguide sensors with synchrotron infrared radiation. Appl Spectrosc 58:143-51
Masip, Lluis; Pan, Jonathan L; Haldar, Suranjana et al. (2004) An engineered pathway for the formation of protein disulfide bonds. Science 303:1185-9
Huang, Raymond Y; Miller, Lisa M; Carlson, Cathy S et al. (2003) In situ chemistry of osteoporosis revealed by synchrotron infrared microspectroscopy. Bone 33:514-21
Rashidzadeh, Hassan; Khrapunov, Sergei; Chance, Mark R et al. (2003) Solution structure and interdomain interactions of the Saccharomyces cerevisiae ""TATA binding protein"" (TBP) probed by radiolytic protein footprinting. Biochemistry 42:3655-65
Uchida, Takeshi; Takamoto, Keiji; He, Qin et al. (2003) Multiple monovalent ion-dependent pathways for the folding of the L-21 Tetrahymena thermophila ribozyme. J Mol Biol 328:463-78
Taylor, Colleen M; Watton, Stephen P; Bryngelson, Peter A et al. (2003) Inner-sphere complexation of cobalt(II) 2,9-dimethyl-1,10-phenanthroline ([Co(neo)]2+) with commercial and sol-gel derived silica gel surfaces. Inorg Chem 42:312-20
Marinkovic, N S; Huang, R; Bromberg, P et al. (2002) Center for Synchrotron Biosciences' U2B beamline: an international resource for biological infrared spectroscopy. J Synchrotron Radiat 9:189-97
Huang, R Y; Miller, L M; Carlson, C S et al. (2002) Characterization of bone mineral composition in the proximal tibia of cynomolgus monkeys: effect of ovariectomy and nandrolone decanoate treatment. Bone 30:492-7
Dewan, John C; Feeling-Taylor, Angela; Puius, Yoram A et al. (2002) Structure of mutant human carbonmonoxyhemoglobin C (betaE6K) at 2.0 A resolution. Acta Crystallogr D Biol Crystallogr 58:2038-42
Kiselar, J G; Maleknia, S D; Sullivan, M et al. (2002) Hydroxyl radical probe of protein surfaces using synchrotron X-ray radiolysis and mass spectrometry. Int J Radiat Biol 78:101-14

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