For the last four years, our group has been investigating structure-function relationship of the catalytic domain of avian sarcoma virus (ASV) integrase. We are interested in detailed structural information on the active and inactive enzyme. For that purpose, three data sets for the wild-type ASV IN and its D64N mutant were collected on beamline X9B. The results are: 1) Native enzyme, crystallized from ammonium sulfate; resolution: 1.02 angstrom; Rmerge: 0.056; Completeness: 100.0%; R and Rfree: 0.190 / 0.211 (refined by X-PLOR). 2) Inactive D64N mutant, low pH; Resolution: 1.20 angstrom; Rmerge: 0.060; Completeness: 97.1%; R and Rfree: 0.193 / 0.229 (refined by X-PLOR) 3) Native enzyme, crystallized from PEG; Resolution: 1.06 angstrom; Rmerge: 0.034; Completeness: 90.1%; R and Rfree: 0.200 / 0.233 (refined by X-PLOR).

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001633-19
Application #
6491514
Study Section
Project Start
2001-09-01
Project End
2002-08-31
Budget Start
Budget End
Support Year
19
Fiscal Year
2001
Total Cost
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461
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