The U.C.S.D. Resource for Protein Crystallography has been extremely successful since its opening seven years ago. Each year we have received, in average, about 20 good proposals from outside users, of which about twelve groups have been given time on the Mark II high-speed data collection systems. During the seven year period, the high intensity measurements for more than 100 different projects. The data have been so good that many of these projects obtained good electron density maps within a few months after the data were collected. During the next five years we would like to expand the resource facilities so that we can better serve the protein crystallography community. First we would like to set up the Mark IV data collection system that can be used to collect data for small crystals or to collect data of crystals with very large unit cell dimensions (like small viruses up to 500 A). This system will have a rotating anode X-ray generator with very high brightness (the Rigaku FR generator). We would like also to continue to explore the idea of using the gold anode to set up a multiwavelength diffractometer that can solve the phase problem by collecting ta on a protein crystal that has been soaked in a platinum reagent. Last, but not least, we would like to explore a completely new way to generate X-rays at different wavelengths using the Compton Backscattering effect.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001644-12
Application #
2281559
Study Section
Special Emphasis Panel (SSS (R6))
Project Start
1983-07-01
Project End
1996-06-30
Budget Start
1994-07-01
Budget End
1995-06-30
Support Year
12
Fiscal Year
1994
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Biology
Type
Schools of Medicine
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Biswas, Saswati; Biswas, Indranil (2011) Role of VltAB, an ABC transporter complex, in viologen tolerance in Streptococcus mutans. Antimicrob Agents Chemother 55:1460-9
Chattoraj, Partho; Banerjee, Anirban; Biswas, Saswati et al. (2010) ClpP of Streptococcus mutans differentially regulates expression of genomic islands, mutacin production, and antibiotic tolerance. J Bacteriol 192:1312-23
Grimplet, Jérôme; Wheatley, Matthew D; Jouira, Hatem Ben et al. (2009) Proteomic and selected metabolite analysis of grape berry tissues under well-watered and water-deficit stress conditions. Proteomics 9:2503-28
Zhang, Jiaqin; Biswas, Indranil (2009) 3'-Phosphoadenosine-5'-phosphate phosphatase activity is required for superoxide stress tolerance in Streptococcus mutans. J Bacteriol 191:4330-40
Drummond, Christopher S (2008) Diversification of Lupinus (Leguminosae) in the western New World: derived evolution of perennial life history and colonization of montane habitats. Mol Phylogenet Evol 48:408-21
Althoff, David M (2008) A test of host-associated differentiation across the 'parasite continuum'in the tri-trophic interaction among yuccas, bogus yucca moths, and parasitoids. Mol Ecol 17:3917-27
Wickstrum, Jason R; Hong, Kee-Jong; Bokhari, Sirosh et al. (2007) Coactivating signals for the hepatic lymphocyte gamma interferon response to Francisella tularensis. Infect Immun 75:1335-42
Hong, Kee-Jong; Wickstrum, Jason R; Yeh, Hung-Wen et al. (2007) Toll-like receptor 2 controls the gamma interferon response to Francisella tularensis by mouse liver lymphocytes. Infect Immun 75:5338-45
Fan, G Y; Datte, P; Beuville, E et al. (1998) ASIC-based event-driven 2D digital electron counter for TEM imaging. Ultramicroscopy 70:107-13
Narayana, N; Cox, S; Nguyen-huu, X et al. (1997) A binary complex of the catalytic subunit of cAMP-dependent protein kinase and adenosine further defines conformational flexibility. Structure 5:921-35

Showing the most recent 10 out of 59 publications