This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Caspase-recruitment doamain (CARD) is an key player of innate immune response and apoptosis. We are studying the molecular mechanism of CARD-mediated protien-protien interactions. We crystallized the CARD of Nod1 protein, which is a cytosolic pathogen sensor. This protien showed a homodimeric conformation. Even though several structures of CARD are available, homodimeric or homooligomeric structure of CARD, which might be a functional form of this protein, is not determined yet. And this crystal diffracted up to 2.4 angstrom on home x-ray source. Since molecular replacement failed due to very low sequence similarity, we are trying to solve the structure with multiple isomorphous replacement.
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