Abstract

The STEM is being used to characterize the reassociated hexagonal bilayer structures obtained from fragments of the hemoglobins of Lumbricus, Tubifex, Arenicola, Amphitrite, Macrobdella and the chlorocruins of Myxicola and Eudistylia. The results of STEM measurements on the reassociated hexagonal bilayer structures have been compared with the proposed """"""""bracelet"""""""" model of the quaternary structure of annelid hemoglobins and chlorocruins and support the model. In this model subunits D1 and D2 form a scaffolding or """"""""bracelet"""""""" decorated with twelve complexes of subunits M and T. In this project the automatic mass program was helpful in recognizing intact top view and side view molecules and measuring masses of thousands of particles (Qabar, Stern, Walz, Jen-Ting, Timovich, Wall, Kapp & Vinogradov, J. Mol. Biol. 222:1109-29, 1991). Similar measurements have been performed on a number of other hemoglobins (Vinogradov, Sharm, Qabar, Wall, Westrick, Simmons & Gill, J. Biol. Chem. 266:13091-96, 1991). A protocol for isolation of earthworm hemoglobin from a live specimen with minimal purification has given molecules of extraordinary quality which we are considering as a new mass standard.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001777-13
Application #
5223604
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
13
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Hind, Geoffrey; Wall, Joseph S; Várkonyi, Zsuzsanna et al. (2014) Membrane crystals of plant light-harvesting complex II disassemble reversibly in light. Plant Cell Physiol 55:1296-303
Garofalo, Robert; Mustanski, Brian; Johnson, Amy et al. (2010) Exploring factors that underlie racial/ethnic disparities in HIV risk among young men who have sex with men. J Urban Health 87:318-323
Joshi, V N; Mitra, D; England, M D et al. (2010) Large Covalently Linked Fluorescent and Gold Nanoparticle Immunoprobes. Microsc Microanal 16:966-967
Ackerson, Christopher J; Powell, Richard D; Hainfeld, James F (2010) Site-specific biomolecule labeling with gold clusters. Methods Enzymol 481:195-230
Hu, Minghui; Qian, Luping; Brinas, Raymond P et al. (2008) Gold nanoparticle-protein arrays improve resolution for cryo-electron microscopy. J Struct Biol 161:83-91
Garofalo, Robert; Mustanski, Brian; Donenberg, Geri (2008) Parents know and parents matter;is it time to develop family-based HIV prevention programs for young men who have sex with men? J Adolesc Health 43:201-4
Brinas, Raymond P; Hu, Minghui; Qian, Luping et al. (2008) Gold nanoparticle size controlled by polymeric Au(I) thiolate precursor size. J Am Chem Soc 130:975-82
Hu, Minghui; Zhang, Yian-Biao; Qian, Luping et al. (2008) Three-dimensional structure of human chromatin accessibility complex hCHRAC by electron microscopy. J Struct Biol 164:263-9
Garofalo, Robert; Mustanski, Brian S; McKirnan, David J et al. (2007) Methamphetamine and young men who have sex with men: understanding patterns and correlates of use and the association with HIV-related sexual risk. Arch Pediatr Adolesc Med 161:591-6
Mustanski, Brian; Garofalo, Robert; Herrick, Amy et al. (2007) Psychosocial health problems increase risk for HIV among urban young men who have sex with men: preliminary evidence of a syndemic in need of attention. Ann Behav Med 34:37-45

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