The Brookhaven STEM (Scanning Transmission Electron Microscopy) Resource provides a unique service for mass and heavy atom cluster mapping of individual biological molecules. STEM I will continue localizing heavy atom clusters and mapping projected mass distribution, giving 1-2 nM useful spatial resolution and 2-10% mass resolution for objects as large as viruses imaged at low dose. STEM III is nearing completion with improved capabilities in resolution, specimen handling and data collection. We propose to continue to expand our productive user/collaborator program as site-specific labeling, specimen preparation, imaging and image analysis methods develop. Currently 65 projects are active in the areas of: protein and protein complexes, filaments and filamentous proteins, membranes and membrane proteins, DNA, RNA and nucleic acid/protein complexes, viral proteins and viruses, heavy atom cluster and cryo electron microscopy. All projects are expected to continue to lead to publications in the highest quality journals. Major emphasis of the core R&D program will continue to be quantitative understanding of specimen preparation as well as development of improved heavy atom cluster labels, improved microscopes, improved imaging techniques (including low dose elemental mapping using specific energy losses) and improved image analysis methods.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001777-17
Application #
2901458
Study Section
Special Emphasis Panel (ZRG2-SSS-3 (34))
Program Officer
Sheeley, Douglas
Project Start
1984-01-01
Project End
2002-03-31
Budget Start
1999-04-01
Budget End
2000-03-31
Support Year
17
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Brookhaven National Laboratory
Department
Type
DUNS #
027579460
City
Upton
State
NY
Country
United States
Zip Code
11973
Hind, Geoffrey; Wall, Joseph S; Várkonyi, Zsuzsanna et al. (2014) Membrane crystals of plant light-harvesting complex II disassemble reversibly in light. Plant Cell Physiol 55:1296-303
Garofalo, Robert; Mustanski, Brian; Johnson, Amy et al. (2010) Exploring factors that underlie racial/ethnic disparities in HIV risk among young men who have sex with men. J Urban Health 87:318-323
Joshi, V N; Mitra, D; England, M D et al. (2010) Large Covalently Linked Fluorescent and Gold Nanoparticle Immunoprobes. Microsc Microanal 16:966-967
Ackerson, Christopher J; Powell, Richard D; Hainfeld, James F (2010) Site-specific biomolecule labeling with gold clusters. Methods Enzymol 481:195-230
Hu, Minghui; Qian, Luping; Brinas, Raymond P et al. (2008) Gold nanoparticle-protein arrays improve resolution for cryo-electron microscopy. J Struct Biol 161:83-91
Garofalo, Robert; Mustanski, Brian; Donenberg, Geri (2008) Parents know and parents matter;is it time to develop family-based HIV prevention programs for young men who have sex with men? J Adolesc Health 43:201-4
Brinas, Raymond P; Hu, Minghui; Qian, Luping et al. (2008) Gold nanoparticle size controlled by polymeric Au(I) thiolate precursor size. J Am Chem Soc 130:975-82
Hu, Minghui; Zhang, Yian-Biao; Qian, Luping et al. (2008) Three-dimensional structure of human chromatin accessibility complex hCHRAC by electron microscopy. J Struct Biol 164:263-9
Garofalo, Robert; Mustanski, Brian S; McKirnan, David J et al. (2007) Methamphetamine and young men who have sex with men: understanding patterns and correlates of use and the association with HIV-related sexual risk. Arch Pediatr Adolesc Med 161:591-6
Mustanski, Brian; Garofalo, Robert; Herrick, Amy et al. (2007) Psychosocial health problems increase risk for HIV among urban young men who have sex with men: preliminary evidence of a syndemic in need of attention. Ann Behav Med 34:37-45

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