Our goal is to determine the coordination environment in the inner coordination sphere of Mn2+ site of FosA protein. The hypothesis is that fosfomycin enters the inner coordination sphere of Mn(2+) where it is activated for nucleophilic attack. We believe that fosfomycin displaces 2 or 3 of the water molecules. Broad Mn(2+) EPR lines observed at 35 GHz preclude the use of 17O-labeling methodology to determine the coordination scheme of Mn(2+) in this complex. With an increase in the magnetic field these lines are getting sharper which allows to use 95 GHz (W-band) EPR to study Mn(2+) coordination.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001811-13
Application #
6120720
Study Section
Project Start
1998-04-15
Project End
1999-11-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
13
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Illinois at Chicago
Department
Type
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612
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