Characterization of active sites in metalloenzymes such as RR (ribonucleotide reductase) and MMO (methane monooxygenase) by electron paramagnetic resonance (EPR) is structurally informative if complete EPR signals can be obtained. But partial EPR signals are found for many high-spin metal ions and metal ion clusters in metalloproteins and model systems. In this case, the method of Hendrich and Debrunner, involving measurement of the partial EPR signals in both normal mode and parallel-mode experiments (oscillating magnetic field) gives the information necessary for quantitation of the signals from the metalloprotein sites and ultimately for testing structural models. This technique is applied to several protein and model system sites. The striking success of this method has stimulated a major instrument supplier, Bruker, to produce parallel-mode accessories for their EPR systems. This is a multi-year ongoing project. 811-12

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001811-13
Application #
6120641
Study Section
Project Start
1998-04-15
Project End
1999-11-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
13
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Illinois at Chicago
Department
Type
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612
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