Abstract

We investigate the fundamental time-resolved fluorescence processes induced by excimer lasers in order to develop quantitative criteria for reliable fluorophore characterization. Our approach involves laser-induced time-resolved fluorescence measurements for the purpose of identification of spectro-temporal patterns characteristic of different fluorophores, that can be used as ``signatures'' for unique and unambiguous characterization. We have been able to obtain such characteristic ``signatures'' in the form of spectro-temporal kernels for various fluorophores (collagen, elastin, NAD and NADH) in vitro, which offers the exciting prospect of unambiguous tissue or compound characterization in vivo, when certain practical impediments are overcome. Samples of human arteries at five different levels of calcification were tested and characterized uniquely by spectro-temporal kernel measurements as a demonstration of the efficacy of this approach. This approach was extended to estimation procedures for fluorescence decay time-constants. The initial results suggest potentially useful clinical implications. The reliability of these measurements in vivo is currently examined in order to assess their clinical applicability.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001861-12
Application #
5223748
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
12
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Song, Dong; Wang, Haonan; Tu, Catherine Y et al. (2013) Identification of sparse neural functional connectivity using penalized likelihood estimation and basis functions. J Comput Neurosci 35:335-57
Hoppenbrouwers, Toke; Oliveira, Flavia; Sandarupa, Stanislaus et al. (2012) The development of the circadian heart rate rhythm (CHR) in Asian infants. Early Hum Dev 88:555-61
Wang, Xiaoning; Schumitzky, Alan; D'Argenio, David Z (2007) Nonlinear Random Effects Mixture Models: Maximum Likelihood Estimation via the EM Algorithm. Comput Stat Data Anal 51:6614-6623
Gholmieh, Ghassan; Courellis, Spiros; Marmarelis, Vasilis et al. (2007) Nonlinear dynamic model of CA1 short-term plasticity using random impulse train stimulation. Ann Biomed Eng 35:847-57
Gholmieh, Ghassan; Courellis, Spiros; Dimoka, Angelika et al. (2004) An algorithm for real-time extraction of population EPSP and population spike amplitudes from hippocampal field potential recordings. J Neurosci Methods 136:111-21
Ashjian, Peter; Elbarbary, Amir; Zuk, Patricia et al. (2004) Noninvasive in situ evaluation of osteogenic differentiation by time-resolved laser-induced fluorescence spectroscopy. Tissue Eng 10:411-20
Blasi, Anna; Jo, Javier; Valladares, Edwin et al. (2003) Cardiovascular variability after arousal from sleep: time-varying spectral analysis. J Appl Physiol 95:1394-404
Jo, Javier A; Blasi, Anna; Valladares, Edwin et al. (2003) Model-based assessment of autonomic control in obstructive sleep apnea syndrome during sleep. Am J Respir Crit Care Med 167:128-36
Belozeroff, Vasily; Berry, Richard B; Khoo, Michael C K (2003) Model-based assessment of autonomic control in obstructive sleep apnea syndrome. Sleep 26:65-73
Bading, James R; Yoo, Paul B; Fissekis, John D et al. (2003) Kinetic modeling of 5-fluorouracil anabolism in colorectal adenocarcinoma: a positron emission tomography study in rats. Cancer Res 63:3667-74

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