Our objectives are to determine the three-dimensional (3D) crystal structures of macromolecules at near atomic resolution and the 3-D structures of non-crystalline macromolecular assemblies at intermediate resolution, by a combination of electron microscopy and computer processing methods. The present proposal is a resource related project. Requests include the acquisition of a medium high voltage (300 kV) electron microscope with appropriate accessories and an operational fund to develop the technology for the above aims. Technical development will be focused on specimen preparation, 3-D data collection at low dose and low temperature conditions and implementation of computer programs for 3-D reconstruction. The developed technology will be immediately applied to the high resolution crystal structure determination of crotoxin complex, T4 DNA helix destabilizing protein, fungus lysozyme and Fc fragments; and to the intermediate resolution structure determination of muscle Z disc and flagella axoneme. Collaborative projects will be established with investigators outside our research groups after the midpoint of the grant period. We plan to complete a thorough analysis of the potential as well as limitations of this technology in its general applicability to a variety of biological systems. We expect to present a final recommendation on the feasibility and desirability of establishing a national shared facility of this technology at the end of this grant period.
| Bucero, Marta Abril; Bajaj, Chandrajit; Mourrain, Bernard (2016) On the construction of general cubature formula by flat extensions. Linear Algebra Appl 502:104-125 |
| Ebeida, Mohamed S; Rushdi, Ahmad A; Awad, Muhammad A et al. (2016) Disk Density Tuning of a Maximal Random Packing. Comput Graph Forum 35:259-269 |
| Wensel, Theodore G; Zhang, Zhixian; Anastassov, Ivan A et al. (2016) Structural and molecular bases of rod photoreceptor morphogenesis and disease. Prog Retin Eye Res 55:32-51 |
| Baker, Mariah R; Fan, Guizhen; Serysheva, Irina I (2015) Single-Particle Cryo-EM of the Ryanodine Receptor Channel in an Aqueous Environment. Eur J Transl Myol 25:4803 |
| Rushdi, Ahmad A; Mitchell, Scott A; Bajaj, Chandrajit L et al. (2015) Robust All-quad Meshing of Domains with Connected Regions. Procedia Eng 124:96-108 |
| Edwards, John; Daniel, Eric; Pascucci, Valerio et al. (2015) Approximating the Generalized Voronoi Diagram of Closely Spaced Objects. Comput Graph Forum 34:299-309 |
| Wensel, Theodore G; Gilliam, Jared C (2015) Three-dimensional architecture of murine rod cilium revealed by cryo-EM. Methods Mol Biol 1271:267-92 |
| Jeter, Cameron B; Patel, Saumil S; Morris, Jeffrey S et al. (2015) Oculomotor executive function abnormalities with increased tic severity in Tourette syndrome. J Child Psychol Psychiatry 56:193-202 |
| Zhang, Qin; Cha, Deukhyun; Bajaj, Chandrajit (2015) Quality Partitioned Meshing of Multi-Material Objects. Procedia Eng 124:187-199 |
| Baker, Mariah R; Fan, Guizhen; Serysheva, Irina I (2015) Single-particle cryo-EM of the ryanodine receptor channel in an aqueous environment. Eur J Transl Myol 25:35-48 |
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