We have developed a computational procedure to animate the 3-dimensional structure of macromolecular assembly determined by electron cryomicroscopy and computer reconstruction. We use the graphics module of EXPLORER software available in SGI workstation to generate the movie frames, which are then transferred to a PC, compressed via commercial proprietary software and written onto a CD. We have found that the CD is conveniently read with a portable CD player which can be connected to a video projector or TV monitor. We have found that using the animation graphics is an effective means to communicate the complicated macromolecular structures.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002250-11
Application #
5223847
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
1996
Total Cost
Indirect Cost
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Ebeida, Mohamed S; Rushdi, Ahmad A; Awad, Muhammad A et al. (2016) Disk Density Tuning of a Maximal Random Packing. Comput Graph Forum 35:259-269
Wensel, Theodore G; Zhang, Zhixian; Anastassov, Ivan A et al. (2016) Structural and molecular bases of rod photoreceptor morphogenesis and disease. Prog Retin Eye Res 55:32-51
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Rushdi, Ahmad A; Mitchell, Scott A; Bajaj, Chandrajit L et al. (2015) Robust All-quad Meshing of Domains with Connected Regions. Procedia Eng 124:96-108
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Wensel, Theodore G; Gilliam, Jared C (2015) Three-dimensional architecture of murine rod cilium revealed by cryo-EM. Methods Mol Biol 1271:267-92
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