Acrosomal bundles from Limulus sperm can be considered as three-dimensional quasi-crystals and reconstructed using a crystallographic approach. However, they are neither """"""""infinitely"""""""" large with respect to the borders of the bundle, nor are they a single unit cell in thickness along the viewing direction. Also, because of their shape, bundles do not generally have a preferred azimuthal orientation, which poses challenges for orientation estimation and refinement. We have developed a strategy for recording and processing electron cryo-microscopic images that differs from classical two-dimensional crystalline reconstruction techniques. These developments allowed us to merge data from tomographic tilt series of ice-embedded acrosomal bundles. The goal is to accurately determine amplitudes and phases at the diffraction maxima in terms of hkl indices, and compute a three-dimensional map from the diffraction data. Our research effort in this project has resulted not only in novel structural information about the bundle but also led to an enhancement of our research infrastructure in terms of experimental and computational capabilities.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
3P41RR002250-17S1
Application #
6611290
Study Section
Project Start
2001-12-01
Project End
2002-11-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
17
Fiscal Year
2002
Total Cost
$134,676
Indirect Cost
Name
Baylor College of Medicine
Department
Type
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030
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